Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4

We describe in this work the identification and the conjugal properties of two cryptic plasmids present in the strain <i>Sinorhizobium meliloti</i> LPU88 isolated from an Argentine soil. One of the plasmids, pSmeLPU88b (22 kb), could be mobilised from different <i>S. meliloti</i...

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Autores principales: Pistorio, Mariano, Del Papa, María Florencia, Balagué, Laura Josefina, Lagares, Antonio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Exactas
Conjugation
Plasmid
Sinorhizobium meliloti
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84577
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84577
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Conjugation
Plasmid
Sinorhizobium meliloti
spellingShingle Ciencias Exactas
Conjugation
Plasmid
Sinorhizobium meliloti
Pistorio, Mariano
Del Papa, María Florencia
Balagué, Laura Josefina
Lagares, Antonio
Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4
topic_facet Ciencias Exactas
Conjugation
Plasmid
Sinorhizobium meliloti
description We describe in this work the identification and the conjugal properties of two cryptic plasmids present in the strain <i>Sinorhizobium meliloti</i> LPU88 isolated from an Argentine soil. One of the plasmids, pSmeLPU88b (22 kb), could be mobilised from different <i>S. meliloti</i> strains to other bacteria by conjugation only if the other plasmid, pSmeLPU88a (139 kb), was present. This latter plasmid, however, could not be transferred via conjugation (frequency <10<SUP>-9</SUP> transconjugants per recipient) contrasting with the conjugal system from the previously described strain GR4, where one plasmid is mobilisable and a second one (helper) is self-transmissible. Despite the differences between the two systems, the conjugative helper functions present in the cryptic plasmids of strain GR4 were active in the mobilisation of plasmid pSmeLPU88b from strain LPU88. Contrasting with this, plasmid pSmeLPU88b was not mobilised by the helper functions of the broad-host-range plasmid RP4. Eckhardt gel analysis showed that none of the plasmids from strain GR4 were excluded in the presence of plasmid pSmeLPU88b suggesting that they all belong to different incompatibility groups for replication. The small plasmid from strain LPU88, pSmeLPU88b, was only able to replicate in members of the Rhizobiaceae family such as <i>Rhizobium leguminosarum</i>, <i>Rhizobium tropici</i> and <i>Agrobacterium tumefaciens</i>, but not in <i>Escherichia coli</i> or <i>Pseudomonas fluorescens</i>. The observation suggests that most likely plasmid pSmeLPU88b was not received from a phylogenetically distant bacterium.
format Articulo
Articulo
author Pistorio, Mariano
Del Papa, María Florencia
Balagué, Laura Josefina
Lagares, Antonio
author_facet Pistorio, Mariano
Del Papa, María Florencia
Balagué, Laura Josefina
Lagares, Antonio
author_sort Pistorio, Mariano
title Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4
title_short Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4
title_full Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4
title_fullStr Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4
title_full_unstemmed Identification of a transmissible plasmid from an Argentine Sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the European strain S. meliloti GR4
title_sort identification of a transmissible plasmid from an argentine sinorhizobium meliloti strain which can be mobilised by conjugative helper functions of the european strain s. meliloti gr4
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84577
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