Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats

We studied the testicular macrophages of rats with experimental autoimmune orchitis (EAO) and analyzed whether the tumor necrosis factor-α (TNFα) is involved in germ cell apoptosis and in Leydig cell steroidogenesis. The EAO was induced in adult male Sprague-Dawley rats by active immunization with t...

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Detalles Bibliográficos
Autores principales: Suescun, María Olga, Rival, Claudia, Theas, María S., Calandra, Ricardo Saúl, Lustig, Livia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2003
Materias:
Ciencias Exactas
Apoptosis
Cytokines
Immunology
Testis
Testosterone
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84565
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84565
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Apoptosis
Cytokines
Immunology
Testis
Testosterone
spellingShingle Ciencias Exactas
Apoptosis
Cytokines
Immunology
Testis
Testosterone
Suescun, María Olga
Rival, Claudia
Theas, María S.
Calandra, Ricardo Saúl
Lustig, Livia
Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
topic_facet Ciencias Exactas
Apoptosis
Cytokines
Immunology
Testis
Testosterone
description We studied the testicular macrophages of rats with experimental autoimmune orchitis (EAO) and analyzed whether the tumor necrosis factor-α (TNFα) is involved in germ cell apoptosis and in Leydig cell steroidogenesis. The EAO was induced in adult male Sprague-Dawley rats by active immunization with testicular homogenate and adjuvants. In the experimental group, a severe orchitis was observed 80 days after the first immunization. ED1- and ED2-positive macrophages were quantified by immunohistochemistry. The TNFα concentration of conditioned media from testicular macrophages (TMCM) was determined by ELISA. The number of apoptotic TNF receptor 1 (TNFR1)-positive germ cells was identified by combining in situ end labeling of apoptotic DNA and immunohistochemical techniques. The effect of TNFα on Leydig cell testosterone production was determined by RIA. In rats with EAO, we observed a significant increase in the number of TNFα-positive testicular macrophages, the TNFα concentration in TMCM, and the number of TNFR1-positive germ cells. Sixty percent of TNFR1-positive germ cells were apoptotic. These results suggest that TNFα could be involved in the pathogenesis of EAO. Acting together with other local factors such as Fas-FasL, TNFα could trigger germ cell apoptosis. We also demonstrated that TNFα inhibited in vitro testosterone production in basal and hCG-stimulated Leydig cells from rats with orchitis.
format Articulo
Articulo
author Suescun, María Olga
Rival, Claudia
Theas, María S.
Calandra, Ricardo Saúl
Lustig, Livia
author_facet Suescun, María Olga
Rival, Claudia
Theas, María S.
Calandra, Ricardo Saúl
Lustig, Livia
author_sort Suescun, María Olga
title Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
title_short Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
title_full Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
title_fullStr Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
title_full_unstemmed Involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
title_sort involvement of tumor necrosis factor-α in the pathogenesis of autoimmune orchitis in rats
publishDate 2003
url http://sedici.unlp.edu.ar/handle/10915/84565
work_keys_str_mv AT suescunmariaolga involvementoftumornecrosisfactorainthepathogenesisofautoimmuneorchitisinrats
AT rivalclaudia involvementoftumornecrosisfactorainthepathogenesisofautoimmuneorchitisinrats
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bdutipo_str Repositorios
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