Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study

A sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of esomeprazole in rabbit plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatogr...

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Autores principales: Zhang, Meiling, Wang, Xuebao, Wang, Zhiyi, Deng, Mingjie, Xu, Zhisheng, Zheng, Yuancai, Zhang, Yuan, Huang, Xueli
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2012
Materias:
Farmacia
Inhibidores de la Bomba de Protones
Espectrometría de Masas
Cromatografía Liquida
Plasma
Conejos
Farmacocinética
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8447
http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_2_6.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8447
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Inhibidores de la Bomba de Protones
Espectrometría de Masas
Cromatografía Liquida
Plasma
Conejos
Farmacocinética
spellingShingle Farmacia
Inhibidores de la Bomba de Protones
Espectrometría de Masas
Cromatografía Liquida
Plasma
Conejos
Farmacocinética
Zhang, Meiling
Wang, Xuebao
Wang, Zhiyi
Deng, Mingjie
Xu, Zhisheng
Zheng, Yuancai
Zhang, Yuan
Huang, Xueli
Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
topic_facet Farmacia
Inhibidores de la Bomba de Protones
Espectrometría de Masas
Cromatografía Liquida
Plasma
Conejos
Farmacocinética
description A sensitive and selective liquid chromatography-mass spectrometry (LC–MS) method for determination of esomeprazole in rabbit plasma was developed and validated. After addition of midazolam as internal standard (IS), protein precipitation by acetonitrile was used as sample preparation, and chromatography involved Agilent SB-C18 column (2.1 x 150 mm, 5.0 μm) using 0.1 % formic acid in water and acetonitrile as a mobile phase with gradient elution. Detection involved positive ion mode electrospray ionization (ESI), and selective ion monitoring (SIM) mode was used for quantification of target fragment ions m/z 198 for esomeprazole and m/z 326 for midazolam (internal standard, IS). The assay was linear over the range of 10–2000 ng/mL for esomeprazole, with a lower limit of quantitation (LLOQ) of 10 ng/mL for esomeprazole. Intra- and inter-day precisions were less than 14 % and the accuracies were in the range of 89.2-112.6 % for esomeprazole. This developed method was successfully applied for the determination of esomeprazole in rabbit plasma for pharmacokinetic study.
format Articulo
Comunicacion
author Zhang, Meiling
Wang, Xuebao
Wang, Zhiyi
Deng, Mingjie
Xu, Zhisheng
Zheng, Yuancai
Zhang, Yuan
Huang, Xueli
author_facet Zhang, Meiling
Wang, Xuebao
Wang, Zhiyi
Deng, Mingjie
Xu, Zhisheng
Zheng, Yuancai
Zhang, Yuan
Huang, Xueli
author_sort Zhang, Meiling
title Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
title_short Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
title_full Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
title_fullStr Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
title_full_unstemmed Determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
title_sort determination of esomeprazole in rabbit plasma by liquid chromatography-mass spectrometry and its application to a pharmacokinetic study
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/8447
http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_2_6.pdf
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