Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense

The interaction between <i>Azospirillum brasilense</i> and plants is not fully understood, although several bacterial surface components like exopolysaccharides (EPS), flagella, and capsular polysaccharides are required for attachment and colonization. While in other plant-bacteria assoc...

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Detalles Bibliográficos
Autores principales:	Jofré, Edgardo, Lagares, Antonio, Mori, Gladys
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2004
Materias:	Ciencias Exactas Azospirillum brasilense dTDP-rhamnose Exopolysaccharide Gas-liquid chromatography-mass spectrometry Lipopolysaccharide
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/84463
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-84463
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Exactas Azospirillum brasilense dTDP-rhamnose Exopolysaccharide Gas-liquid chromatography-mass spectrometry Lipopolysaccharide
spellingShingle	Ciencias Exactas Azospirillum brasilense dTDP-rhamnose Exopolysaccharide Gas-liquid chromatography-mass spectrometry Lipopolysaccharide Jofré, Edgardo Lagares, Antonio Mori, Gladys Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
topic_facet	Ciencias Exactas Azospirillum brasilense dTDP-rhamnose Exopolysaccharide Gas-liquid chromatography-mass spectrometry Lipopolysaccharide
description	The interaction between <i>Azospirillum brasilense</i> and plants is not fully understood, although several bacterial surface components like exopolysaccharides (EPS), flagella, and capsular polysaccharides are required for attachment and colonization. While in other plant-bacteria associations (<i>Rhizobium</i>-legume, <i>Pseudomonas</i>-potato), lipopolysaccharides (LPS) play a key role in the establishment of an effective association, their role in the root colonization by Azospirillum had not been determined. In this study, we isolated a Tn5 mutant of <i>A. brasilense</i> Cd (EJ1) with an apparently modified LPS core structure, non-mucoid colony morphology, increased EPS production, and affected in maize root colonization. A 3790-bp region revealed the presence of three complete open reading frames designated <i>rmlC</i>, <i>rmlB</i> and <i>rmlD</i>. The beginning of a fourth open reading frame was found and designated rmlA. These genes are organized in a cluster which shows homology to the cluster involved in the synthesis of dTDP-rhamnose in other bacteria. Additionally, the analysis of the monosaccharide composition of LPSs showed a diminution of rhamnose compared to the wild-type strain.
format	Articulo Articulo
author	Jofré, Edgardo Lagares, Antonio Mori, Gladys
author_facet	Jofré, Edgardo Lagares, Antonio Mori, Gladys
author_sort	Jofré, Edgardo
title	Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
title_short	Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
title_full	Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
title_fullStr	Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
title_full_unstemmed	Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense
title_sort	disruption of dtdp-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in azospirillum brasilense
publishDate	2004
url	http://sedici.unlp.edu.ar/handle/10915/84463
work_keys_str_mv	AT jofreedgardo disruptionofdtdprhamnosebiosynthesismodifieslipopolysaccharidecoreexopolysaccharideproductionandrootcolonizationinazospirillumbrasilense AT lagaresantonio disruptionofdtdprhamnosebiosynthesismodifieslipopolysaccharidecoreexopolysaccharideproductionandrootcolonizationinazospirillumbrasilense AT morigladys disruptionofdtdprhamnosebiosynthesismodifieslipopolysaccharidecoreexopolysaccharideproductionandrootcolonizationinazospirillumbrasilense
bdutipo_str	Repositorios
_version_	1764820488764260356

Disruption of dTDP-rhamnose biosynthesis modifies lipopolysaccharide core, exopolysaccharide production, and root colonization in Azospirillum brasilense

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