Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin

Diffractaic acid (DA) presents several biological activities. The goal of this study was to develop and validate a UV spectrophotometric method for determining diffractaic acid in inclusion complexes with hydroxypropyl-β-cyclodextrin. Validation parameters were determined according to international...

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Detalles Bibliográficos
Autores principales:	Silva, Camilla V.N.S., Santos, Noemia P. S., Santos Magalhães, Nereide S., Lira, Mariane C. B., Honda, Neli Kika, Ferraz, Milena S.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2012
Materias:	Farmacia Ácidos diffractaic acid; hydroxypropyl-β-cyclodextrin; inclusion complex; validation method Plantas Medicinales
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8438 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_18.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8438
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Ácidos diffractaic acid; hydroxypropyl-β-cyclodextrin; inclusion complex; validation method Plantas Medicinales
spellingShingle	Farmacia Ácidos diffractaic acid; hydroxypropyl-β-cyclodextrin; inclusion complex; validation method Plantas Medicinales Silva, Camilla V.N.S. Santos, Noemia P. S. Santos Magalhães, Nereide S. Lira, Mariane C. B. Honda, Neli Kika Ferraz, Milena S. Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
topic_facet	Farmacia Ácidos diffractaic acid; hydroxypropyl-β-cyclodextrin; inclusion complex; validation method Plantas Medicinales
description	Diffractaic acid (DA) presents several biological activities. The goal of this study was to develop and validate a UV spectrophotometric method for determining diffractaic acid in inclusion complexes with hydroxypropyl-β-cyclodextrin. Validation parameters were determined according to international guidelines for standardization. The linearity range of analytical curve was from 1 to 5 μg/mL and the regression equation: CDA = (Area - 0.0053)/0.1541 (r2 = 0.99998; n = 3). The intermediate precision indicated that the difference between the means was statistically insignificant (p < 0.05). Accuracy revealed a mean recovery percentage of diffractaic acid in inclusion complexes of 100.1 %. The method was robust and the formulation excipients did not interfere on diffractaic acid quantification. Limits of detection and quantification of diffractaic acid were 0.03 and 0.08 μg/mL, respectively. The proposed method proved to be accurate, precise and reproducible, thus being able to quantify diffractaic acid in raw material and inclusion complexes.
format	Articulo Articulo
author	Silva, Camilla V.N.S. Santos, Noemia P. S. Santos Magalhães, Nereide S. Lira, Mariane C. B. Honda, Neli Kika Ferraz, Milena S.
author_facet	Silva, Camilla V.N.S. Santos, Noemia P. S. Santos Magalhães, Nereide S. Lira, Mariane C. B. Honda, Neli Kika Ferraz, Milena S.
author_sort	Silva, Camilla V.N.S.
title	Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_short	Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_full	Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_fullStr	Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_full_unstemmed	Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
title_sort	validation of an uv spectrophotometric method for determining diffractaic acid from usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin
publishDate	2012
url	http://sedici.unlp.edu.ar/handle/10915/8438 http://www.latamjpharm.org/resumenes/31/1/LAJOP_31_1_1_18.pdf
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Validation of an UV spectrophotometric method for determining diffractaic acid from Usnea sp. in inclusion complexes with hydroxypropyl-β-cyclodextrin

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