Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti

Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of <i>Sinorhizobium meliloti</i>. An <i>S...

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Autores principales: Harrison, Judith, Jamet, Alexandre, Muglia, Cecilia Isabel, Van de Sype, Ghislaine, Aguilar, Orlando Mario, Puppo, Alain, Frendo, Pierre
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Ciencias Exactas
Sinorhizobium meliloti
glutathione
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84294
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84294
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Sinorhizobium meliloti
glutathione
spellingShingle Ciencias Exactas
Sinorhizobium meliloti
glutathione
Harrison, Judith
Jamet, Alexandre
Muglia, Cecilia Isabel
Van de Sype, Ghislaine
Aguilar, Orlando Mario
Puppo, Alain
Frendo, Pierre
Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti
topic_facet Ciencias Exactas
Sinorhizobium meliloti
glutathione
description Rhizobia form a symbiotic relationship with plants of the legume family to produce nitrogen-fixing root nodules under nitrogen-limiting conditions. We have examined the importance of glutathione (GSH) during free-living growth and symbiosis of <i>Sinorhizobium meliloti</i>. An <i>S. meliloti</i> mutant strain (SmgshA) which is unable to synthesize GSH due to a gene disruption in <i>gshA</i>, encoding the enzyme for the first step in the biosynthesis of GSH, was unable to grow under nonstress conditions, precluding any nodulation. In contrast, an <i>S. meliloti</i> strain (SmgshB) with <i>gshB</i>, encoding the enzyme involved in the second step in GSH synthesis, deleted was able to grow, indicating that γ- glutamylcysteine, the dipeptide intermediate, can partially substitute for GSH. However, the SmgshB strain showed a delayed-nodulation phenotype coupled to a 75% reduction in the nitrogen fixation capacity. This phenotype was linked to abnormal nodule development. Both the SmgshA and SmgshB mutant strains exhibited higher catalase activity than the wild-type <i>S. meliloti</i> strain, suggesting that both mutant strains are under oxidative stress. Taken together, these results show that GSH plays a critical role in the growth of <i>S. meliloti</i> and during its interaction with the plant partner.
format Articulo
Articulo
author Harrison, Judith
Jamet, Alexandre
Muglia, Cecilia Isabel
Van de Sype, Ghislaine
Aguilar, Orlando Mario
Puppo, Alain
Frendo, Pierre
author_facet Harrison, Judith
Jamet, Alexandre
Muglia, Cecilia Isabel
Van de Sype, Ghislaine
Aguilar, Orlando Mario
Puppo, Alain
Frendo, Pierre
author_sort Harrison, Judith
title Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti
title_short Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti
title_full Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti
title_fullStr Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti
title_full_unstemmed Glutathione plays a fundamental role in growth and symbiotic capacity of Sinorhizobium meliloti
title_sort glutathione plays a fundamental role in growth and symbiotic capacity of sinorhizobium meliloti
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/84294
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