Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD

The objective of this paper was to develop a high performance liquid chromatography with diode array detection both for chromatographic fingerprint and simultaneous determination of twelve analytes of Shuang-Huang-Lian (SHL) capsule. The chromatographic separation was performed on an Aglient Zorbax...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Zhao, Ziming, Jiang, Xianglan, Yin, Xiaoxin, Tang, Daoquan, An, Yi-qiang, Chen, Xu, Wu, Jing
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
fingerprint analysis; high performance liquid chromatography; multiple compounds quantification; Shuang-Huang-Lian capsule
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8415
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_27.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8415
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
fingerprint analysis; high performance liquid chromatography; multiple compounds quantification; Shuang-Huang-Lian capsule
spellingShingle Farmacia
fingerprint analysis; high performance liquid chromatography; multiple compounds quantification; Shuang-Huang-Lian capsule
Zhao, Ziming
Jiang, Xianglan
Yin, Xiaoxin
Tang, Daoquan
An, Yi-qiang
Chen, Xu
Wu, Jing
Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD
topic_facet Farmacia
fingerprint analysis; high performance liquid chromatography; multiple compounds quantification; Shuang-Huang-Lian capsule
description The objective of this paper was to develop a high performance liquid chromatography with diode array detection both for chromatographic fingerprint and simultaneous determination of twelve analytes of Shuang-Huang-Lian (SHL) capsule. The chromatographic separation was performed on an Aglient Zorbax SB-C<sub>18</sub> column with a gradient elution program using a mixture of acetonitrile and 0.2 % acetic acid as mobile phase within 110 min detected at 278 nm wavelength. For fingerprint analysis, 50 peaks were selected as the common peaks to evaluate the similarities of different samples collected from different pharmaceutical companies in China, and two kinds of data, relative retention time and relative peak area were used to identify the common peaks in samples for investigation. SHL capsules from different batches of the same manufacturer or different manufacturers showed a close similarity. For quantitative analysis, linear regressions, limit of detection and quantification, intra-day and inter-day precisions, recovery, repeatability and stability were all tested and good results were obtained to simultaneously determine the 12 marker compounds in the samples. The validated method coupled with multiple compounds determination and fingerprint analysis is a powerful and meaningful tool to comprehensively conduct the quality control of TCM.
format Articulo
Articulo
author Zhao, Ziming
Jiang, Xianglan
Yin, Xiaoxin
Tang, Daoquan
An, Yi-qiang
Chen, Xu
Wu, Jing
author_facet Zhao, Ziming
Jiang, Xianglan
Yin, Xiaoxin
Tang, Daoquan
An, Yi-qiang
Chen, Xu
Wu, Jing
author_sort Zhao, Ziming
title Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD
title_short Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD
title_full Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD
title_fullStr Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD
title_full_unstemmed Multiple compounds determination and fingerprint analysis of herbal preparation Shuang-Huang-Lian capsule by HPLC-DAD
title_sort multiple compounds determination and fingerprint analysis of herbal preparation shuang-huang-lian capsule by hplc-dad
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8415
http://www.latamjpharm.org/resumenes/30/10/LAJOP_30_10_1_27.pdf
work_keys_str_mv AT zhaoziming multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
AT jiangxianglan multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
AT yinxiaoxin multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
AT tangdaoquan multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
AT anyiqiang multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
AT chenxu multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
AT wujing multiplecompoundsdeterminationandfingerprintanalysisofherbalpreparationshuanghuangliancapsulebyhplcdad
bdutipo_str Repositorios
_version_ 1764820488422424579

Ejemplares similares