Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen

Whooping cough is a reemerging disease caused by two closely related pathogens, <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i>. The incidence of <i>B. parapertussis</i> in whooping cough cases has been increasing since the introduction of acellula...

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Autores principales: Gorgojo, Juan Pablo, Lamberti, Yanina Andrea, Valdez, Hugo Alberto, Harvill, Eric T., Rodríguez, María Eugenia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2012
Materias:
Ciencias Exactas
Bordetella parapertussis
Lipopolysaccharide O Antigen
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/84072
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-84072
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Bordetella parapertussis
Lipopolysaccharide O Antigen
spellingShingle Ciencias Exactas
Bordetella parapertussis
Lipopolysaccharide O Antigen
Gorgojo, Juan Pablo
Lamberti, Yanina Andrea
Valdez, Hugo Alberto
Harvill, Eric T.
Rodríguez, María Eugenia
Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen
topic_facet Ciencias Exactas
Bordetella parapertussis
Lipopolysaccharide O Antigen
description Whooping cough is a reemerging disease caused by two closely related pathogens, <i>Bordetella pertussis</i> and <i>Bordetella parapertussis</i>. The incidence of <i>B. parapertussis</i> in whooping cough cases has been increasing since the introduction of acellular pertussis vaccines containing purified antigens that are common to both strains. Recently published results demonstrated that these vaccines do not protect against <i>B. parapertussis</i> due to the presence of the O antigen on the bacterial surface that impairs antibody access to shared antigens. We have investigated the effect of the lack of opsonization of <i>B. parapertussis</i> on the outcome of its interaction with human neutrophils (polymorphonuclear leukocytes [PMNs]). In the absence of opsonic antibodies, PMN interaction with <i>B. parapertussis</i> resulted in nonbactericidal trafficking upon phagocytosis. A high percentage of nonopsonized <i>B. parapertussis</i> was found in nonacidic lysosome marker (lysosome-associated membrane protein [LAMP])-negative phagosomes with access to the host cell-recycling pathway of external nutrients, allowing bacterial survival as determined by intracellular CFU counts. The lipopolysaccharide (LPS) O antigen was found to be involved in directing <i>B. parapertussis</i> to PMN lipid rafts, eventually determining the nonbactericidal fate inside the PMN. IgG opsonization of <i>B. parapertussis</i> drastically changed this interaction by not only inducing efficient PMN phagocytosis but also promoting PMN bacterial killing. These data provide new insights into the immune mechanisms of hosts against <i>B. parapertussis</i> and document the crucial importance of opsonic antibodies in immunity to this pathogen.
format Articulo
Articulo
author Gorgojo, Juan Pablo
Lamberti, Yanina Andrea
Valdez, Hugo Alberto
Harvill, Eric T.
Rodríguez, María Eugenia
author_facet Gorgojo, Juan Pablo
Lamberti, Yanina Andrea
Valdez, Hugo Alberto
Harvill, Eric T.
Rodríguez, María Eugenia
author_sort Gorgojo, Juan Pablo
title Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen
title_short Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen
title_full Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen
title_fullStr Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen
title_full_unstemmed Bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide O antigen
title_sort bordetella parapertussis survives the innate interaction with human neutrophils by impairing bactericidal trafficking inside the cell through a lipid raft-dependent mechanism mediated by the lipopolysaccharide o antigen
publishDate 2012
url http://sedici.unlp.edu.ar/handle/10915/84072
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