Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor
The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR m...
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Autores principales: | , , , , , , , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
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2008
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83531 |
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I19-R120-10915-83531 |
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institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Química Ca2+-sensing receptor Cardiac function Cardiac microvascular endothelial cells Extracellular Ca2+ Fura-2/AM Inositol 1,4,5-trisphosphate Na+/Ca2+ exchanger Phospholipase C Vascular tone |
spellingShingle |
Química Ca2+-sensing receptor Cardiac function Cardiac microvascular endothelial cells Extracellular Ca2+ Fura-2/AM Inositol 1,4,5-trisphosphate Na+/Ca2+ exchanger Phospholipase C Vascular tone Berra Romani, Roberto Raqeeb, Abdul Laforenza, Umberto Scaffino, Manuela Federica Moccia, Francesco Avelino-Cruz, Josè Everardo Oldani, Amanda Coltrini, Daniela Milesi, Verónica Taglietti, Vanni Tanzi, Franco Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
topic_facet |
Química Ca2+-sensing receptor Cardiac function Cardiac microvascular endothelial cells Extracellular Ca2+ Fura-2/AM Inositol 1,4,5-trisphosphate Na+/Ca2+ exchanger Phospholipase C Vascular tone |
description |
The mechanism whereby extracellular Ca2+ exerts the endothelium-dependent control of vascular tone is still unclear. In this study, we assessed whether cardiac microvascular endothelial cells (CMEC) express a functional extracellular Ca2+-sensing receptor (CaSR) using a variety of techniques. CaSR mRNA was detected using RT-PCR, and CaSR protein was identified by immunocytochemical analysis. In order to assess the functionality of the receptor, CMEC were loaded with the Ca2+-sensitive fluorochrome, Fura-2/AM. A number of CaSR agonists, such as spermine, Gd 3+, La3+ and neomycin, elicited a heterogeneous intracellular Ca2+ signal, which was abolished by disruption of inositol 1,4,5-trisphosphate (InsP3) signaling and by depletion of intracellular stores with cyclopiazonic acid. The inhibition of the Na +/Ca2+ exchanger upon substitution of extracellular Na+ unmasked the Ca2+ signal triggered by an increase in extracellular Ca2+ levels. Finally, aromatic amino acids, which function as allosteric activators of CaSR, potentiated the Ca2+ response to the CaSR agonist La3+. These data provide evidence that CMEC express CaSR, which is able to respond to physiological agonists by mobilizing Ca2+ from intracellular InsP3-sensitive stores. |
format |
Articulo Articulo |
author |
Berra Romani, Roberto Raqeeb, Abdul Laforenza, Umberto Scaffino, Manuela Federica Moccia, Francesco Avelino-Cruz, Josè Everardo Oldani, Amanda Coltrini, Daniela Milesi, Verónica Taglietti, Vanni Tanzi, Franco |
author_facet |
Berra Romani, Roberto Raqeeb, Abdul Laforenza, Umberto Scaffino, Manuela Federica Moccia, Francesco Avelino-Cruz, Josè Everardo Oldani, Amanda Coltrini, Daniela Milesi, Verónica Taglietti, Vanni Tanzi, Franco |
author_sort |
Berra Romani, Roberto |
title |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_short |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_full |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_fullStr |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_full_unstemmed |
Cardiac microvascular endothelial cells express a functional Ca 2+-sensing receptor |
title_sort |
cardiac microvascular endothelial cells express a functional ca 2+-sensing receptor |
publishDate |
2008 |
url |
http://sedici.unlp.edu.ar/handle/10915/83531 |
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Repositorios |
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