Heterogeneity of circulating prolactin in the bitch

Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with hig...

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Detalles Bibliográficos
Autores principales: Gobello, María Cristina, Colombani, Miriam, Scaglia, Hugo, Sota, Rodolfo Luzbel de la, Goya, Rodolfo Gustavo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2001
Materias:
Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83506
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83506
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
spellingShingle Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
Gobello, María Cristina
Colombani, Miriam
Scaglia, Hugo
Sota, Rodolfo Luzbel de la
Goya, Rodolfo Gustavo
Heterogeneity of circulating prolactin in the bitch
topic_facet Ciencias Veterinarias
Bitch
Diestrus
Molecular variants
Prolactin
Pseudopregnancy
description Different molecular forms of circulating prolactin (PRL) are known to occur in several species. As no such information was available in dogs, we assessed the molecular profile of circulating PRL in bitches. Pooled sera from covertly (CTRL) and overtly pseudopregnant (PSPT) diestrous bitches with high or low (> 10 or < 10 ng·mL<SUP>-1</SUP>, respectively) serum PRL (measured by ELISA) were analyzed by Sephadex G-100 and Concanavalin A-Sepharose column chromatography. Four serum PRL fractions were identified and termed big-big, big (> 67 kDa), native (23 kDa) and fragmented (< 20 kDa) PRL. The percentages of these fractions were roughly similar in CTRL and PSPT animals, irrespective of their serum PRL levels (higher in PSPT than in CTRL bitches). A large proportion of glycosylated PRL (between 69 and 100%) was also detected in these sera. We conclude that in dogs, circulating PRL occurs in multiple molecular forms, whose relative abundance is comparable in covertly and overtly pseudopregnant bitches.
format Articulo
Articulo
author Gobello, María Cristina
Colombani, Miriam
Scaglia, Hugo
Sota, Rodolfo Luzbel de la
Goya, Rodolfo Gustavo
author_facet Gobello, María Cristina
Colombani, Miriam
Scaglia, Hugo
Sota, Rodolfo Luzbel de la
Goya, Rodolfo Gustavo
author_sort Gobello, María Cristina
title Heterogeneity of circulating prolactin in the bitch
title_short Heterogeneity of circulating prolactin in the bitch
title_full Heterogeneity of circulating prolactin in the bitch
title_fullStr Heterogeneity of circulating prolactin in the bitch
title_full_unstemmed Heterogeneity of circulating prolactin in the bitch
title_sort heterogeneity of circulating prolactin in the bitch
publishDate 2001
url http://sedici.unlp.edu.ar/handle/10915/83506
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AT colombanimiriam heterogeneityofcirculatingprolactininthebitch
AT scagliahugo heterogeneityofcirculatingprolactininthebitch
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