Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors

The aim of this work was to study the possible relationship between pancreatic duodenal homeobox-1 (Pdx-1) and islet neogenesis-associated protein (INGAP) during induced islet neogenesis. Pregnant hamsters were fed with (S) and without (C) sucrose, and glycemia, insulin secretion <i>in vitro&l...

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Autores principales:	Gagliardino, Juan José, Del Zotto, Héctor Herminio, Massa, María Laura, Flores, Luis Emilio, Borelli, María Inés
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2003
Materias:	Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/83428
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-83428
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis
spellingShingle	Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis Gagliardino, Juan José Del Zotto, Héctor Herminio Massa, María Laura Flores, Luis Emilio Borelli, María Inés Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
topic_facet	Ciencias Médicas pancreatic duodenal homeobox-1 islet neogenesis-associated protein neogenesis
description	The aim of this work was to study the possible relationship between pancreatic duodenal homeobox-1 (Pdx-1) and islet neogenesis-associated protein (INGAP) during induced islet neogenesis. Pregnant hamsters were fed with (S) and without (C) sucrose, and glycemia, insulin secretion <i>in vitro</i>, and pancreas immunomorphometric parameters were measured in their 7-day-old offspring. S offspring had significantly lower glycemic levels than C animals. Insulin release in response to increasing glucose contrations in the incubation medium (2-16 mM glucose) did not increase in pancreata from either C or S offspring. However, pancreata from S offspring released more insulin than those from C animals. In S offspring, β-cell mass, β-cell replication rate and islet neogenesis increased significantly, with a simultaneous decrease in β-cell apoptotic rate. INGAP- and Pdx-1-positive cell mass also increased in the islets and among acinar and duct cells. We found two subpopulations of Pdx-1 cells: INGAP-positive and INGAP-negative. Pdx-1/INGAP-positive cells did not stain with insulin, glucagon, somatostatin, pancreatic polypeptide, or neurogenin 3 antibodies. The increment of Pdx-1/INGAP-positive cells represented the major contribution to the Pdx-1 cell mass increase. Such increments varied among pancreas subsectors: ductal>insular>extrainsular. Our results suggested that INGAP participates in the regulation of islet neogenesis, and Pdx-1/INGAP-positive cells represent a new stem cell subpopulation at an early stage of development, highly activateable in neogenesis.
format	Articulo Articulo
author	Gagliardino, Juan José Del Zotto, Héctor Herminio Massa, María Laura Flores, Luis Emilio Borelli, María Inés
author_facet	Gagliardino, Juan José Del Zotto, Héctor Herminio Massa, María Laura Flores, Luis Emilio Borelli, María Inés
author_sort	Gagliardino, Juan José
title	Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
title_short	Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
title_full	Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
title_fullStr	Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
title_full_unstemmed	Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors
title_sort	pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: a possible combined marker of activateable pancreatic cell precursors
publishDate	2003
url	http://sedici.unlp.edu.ar/handle/10915/83428
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bdutipo_str	Repositorios
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Pancreatic duodenal homeobox-1 and islet neogenesis-associated protein: A possible combined marker of activateable pancreatic cell precursors

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