Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers

The effects of the organophosphorous insecticide fenitrothion (phosphorothioic acid, O,O-dimethyl O-(3-methyl-4-nitrophenyl) ester; FS) on the physical state of pure dipalmitoyl (DPPC) and 1-palmitoyl-2- oleoylphosphatidylcholine (POPC) membranes were investigated. FS lowers the phase transition tem...

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Detalles Bibliográficos
Autores principales: González Baró, María del Rosario, Garda, Horacio Alberto, Pollero, Ricardo José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2000
Materias:
Ciencias Médicas
Ciencias Exactas
Fenitrothion
Fluorescence diffusion
Fluorescence quenching
Lipid bilayers
Lipid phase behaviour
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83422
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83422
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Ciencias Exactas
Fenitrothion
Fluorescence diffusion
Fluorescence quenching
Lipid bilayers
Lipid phase behaviour
spellingShingle Ciencias Médicas
Ciencias Exactas
Fenitrothion
Fluorescence diffusion
Fluorescence quenching
Lipid bilayers
Lipid phase behaviour
González Baró, María del Rosario
Garda, Horacio Alberto
Pollero, Ricardo José
Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
topic_facet Ciencias Médicas
Ciencias Exactas
Fenitrothion
Fluorescence diffusion
Fluorescence quenching
Lipid bilayers
Lipid phase behaviour
description The effects of the organophosphorous insecticide fenitrothion (phosphorothioic acid, O,O-dimethyl O-(3-methyl-4-nitrophenyl) ester; FS) on the physical state of pure dipalmitoyl (DPPC) and 1-palmitoyl-2- oleoylphosphatidylcholine (POPC) membranes were investigated. FS lowers the phase transition temperature of DPPC. It has no large effects on the DPPC gel phase, but it increases the order of the liquid-crystalline state of DPPC and POPC. FS also decreases 1,6-diphenyl-1,3,5-hexatriene (DPH) lifetime (τ) in the DPPC and POPC liquid-crystalline states. Since a direct quenching of DPH emission by FS was ruled out, τ shortening is assigned to an increased water penetration in the bilayer. The effect of FS is different from most perturbing agents for which an increased order is accompanied by a higher τ. Furthermore, quenching of DPH by KI was increased by FS in POPC liposomes indicating an increased accessibility of the quencher to the hydrophobic core where DPH distributes. The effect of FS on dipole relaxation at the hydrophilic-hydrophobic interface of POPC bilayers was studied with 2- dimethylamino-6-lauroylnaphthalene (Laurdan). FS produces a decrease in Laurdan τ and a narrowing of its emission band. FS significantly increases the generalized polarization values at both emission band ends. These results indicate that FS may allow the coexistence of microdomains that have different physical properties.
format Articulo
Articulo
author González Baró, María del Rosario
Garda, Horacio Alberto
Pollero, Ricardo José
author_facet González Baró, María del Rosario
Garda, Horacio Alberto
Pollero, Ricardo José
author_sort González Baró, María del Rosario
title Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
title_short Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
title_full Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
title_fullStr Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
title_full_unstemmed Effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
title_sort effect of fenitrothion on dipalmitoyl and 1-palmitoyl-2-oleoylphosphatidylcholine bilayers
publishDate 2000
url http://sedici.unlp.edu.ar/handle/10915/83422
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AT gardahoracioalberto effectoffenitrothionondipalmitoyland1palmitoyl2oleoylphosphatidylcholinebilayers
AT polleroricardojose effectoffenitrothionondipalmitoyland1palmitoyl2oleoylphosphatidylcholinebilayers
bdutipo_str Repositorios
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