Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system

Besides the hypothalamus and pituitary, melatonin action at the testicuiar level has been recently suggested. Therefore, we investigated in the Syrian hamster, a well-characterized seasonal breeder, melatonin action on Leydig cells, testicular expression of melatonergic receptors, and possible inter...

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Autores principales: Frungieri, Mónica Beatriz, Mayerhofer, Artur, Zitta, Karina, Pignataro, Omar P., Calandra, Ricardo Saúl, Gonzalez-Calvar, Silvia I.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2005
Materias:
Biología
melatonin
Leydig cells
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83416
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83416
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
melatonin
Leydig cells
spellingShingle Biología
melatonin
Leydig cells
Frungieri, Mónica Beatriz
Mayerhofer, Artur
Zitta, Karina
Pignataro, Omar P.
Calandra, Ricardo Saúl
Gonzalez-Calvar, Silvia I.
Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
topic_facet Biología
melatonin
Leydig cells
description Besides the hypothalamus and pituitary, melatonin action at the testicuiar level has been recently suggested. Therefore, we investigated in the Syrian hamster, a well-characterized seasonal breeder, melatonin action on Leydig cells, testicular expression of melatonergic receptors, and possible interactions between melatonin receptors and the previously identified testicular serotoninergic and CRH systems. In isolated Leydig cells from active testes of adult hamsters kept in a long-day (14 h light, 10 h dark) photoperiod and from regressed testes of adult animals exposed to a short-day photoperiod during 16 wk (6 h light, 18 h dark), melatonin significantly reduced human chorionic gonadotropin-stimulated production of cAMP and the main androgens: testosterone and androstane-3α,17β-diol, respectively, and decreased the expression of steroidogenic acute regulatory protein, P450 side chain cleavage, 3β-hydroxysteroid dehydrogenase and 17β-hydroxysteroid dehydrogenase. In Leydig cells exposed to a short-day photoperiod during 16 wk, melatonin stimulated the conversion of testosterone into 5α-reduced androgens by inducing 5α-reductase isoform 1, and controlled androstane-3α,17β-diol production by inhibiting 3α- hydroxysteroid dehydrogenase expression. Melatonin subtype (mella) receptors were detected in Leydig cells. Although the local serotonin system did not mediate melatonin action on androgen production, melatonergic effect on steroidogenesis involved the interaction between mella receptors and the inhibitory CEH system. Moreover, melatonin significantly increased CRH mRNA levels and production in hamster Leydig cells expressing CRH subtype 1 receptors. Our studies indicate that melatonin may act as a local inhibitor of human chorionic gonadotropin-stimulated cAMP and androgen production through mella receptors, down-regulation of steroidogenic acute regulatory protein, and key steroidogenic enzymes expression and its interaction with the local CRH system.
format Articulo
Articulo
author Frungieri, Mónica Beatriz
Mayerhofer, Artur
Zitta, Karina
Pignataro, Omar P.
Calandra, Ricardo Saúl
Gonzalez-Calvar, Silvia I.
author_facet Frungieri, Mónica Beatriz
Mayerhofer, Artur
Zitta, Karina
Pignataro, Omar P.
Calandra, Ricardo Saúl
Gonzalez-Calvar, Silvia I.
author_sort Frungieri, Mónica Beatriz
title Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
title_short Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
title_full Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
title_fullStr Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
title_full_unstemmed Direct effect of melatonin on Syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
title_sort direct effect of melatonin on syrian hamster testes: melatonin subtype 1a receptors, inhibition of androgen production, and interaction with the local corticotropin-releasing hormone system
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83416
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