Isolation of equine herpesvirus-2 from the lung of an aborted fetus

This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclona...

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Autores principales: Galosi, Cecilia Mónica, Cid de La Paz, Viviana, Fernández, Leandro Carlos, Martínez, Javier Pablo, Craig, María Isabel, Barrandeguy, María, Etcheverrigaray, María Elisa
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2005
Materias:
Ciencias Veterinarias
Aborted fetus
Equine herpesvirus-2
Isolation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83372
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83372
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
Aborted fetus
Equine herpesvirus-2
Isolation
spellingShingle Ciencias Veterinarias
Aborted fetus
Equine herpesvirus-2
Isolation
Galosi, Cecilia Mónica
Cid de La Paz, Viviana
Fernández, Leandro Carlos
Martínez, Javier Pablo
Craig, María Isabel
Barrandeguy, María
Etcheverrigaray, María Elisa
Isolation of equine herpesvirus-2 from the lung of an aborted fetus
topic_facet Ciencias Veterinarias
Aborted fetus
Equine herpesvirus-2
Isolation
description This study describes the isolation of equine herpesvirus-2 (EHV-2) from the lung of an aborted equine fetus in Argentina. The isolated virus was confirmed as EHV-2 by indirect immunofluorescence using a rabbit anti-EHV-2 polyclonal antiserum and by virus-neutralization test using an equine polyclonal antibody against EHV-2. Restriction endonuclease DNA fingerprinting with BamHI also confirmed the identity of the virus as EHV-2. Furthermore, viral nucleic acid was detected by polymerase chain reaction from the original lung sample and from the DNA obtained from cells infected with the virus isolate. This work constitutes the first reported isolation of EHV-2 from an aborted equine fetus. The presence of EHV-2 in the lung of the aborted fetus would indicate that this virus is capable of crossing the placental barrier. However, no cause-effect relationship was established between the EHV-2 isolate and the abortion.
format Articulo
Comunicacion
author Galosi, Cecilia Mónica
Cid de La Paz, Viviana
Fernández, Leandro Carlos
Martínez, Javier Pablo
Craig, María Isabel
Barrandeguy, María
Etcheverrigaray, María Elisa
author_facet Galosi, Cecilia Mónica
Cid de La Paz, Viviana
Fernández, Leandro Carlos
Martínez, Javier Pablo
Craig, María Isabel
Barrandeguy, María
Etcheverrigaray, María Elisa
author_sort Galosi, Cecilia Mónica
title Isolation of equine herpesvirus-2 from the lung of an aborted fetus
title_short Isolation of equine herpesvirus-2 from the lung of an aborted fetus
title_full Isolation of equine herpesvirus-2 from the lung of an aborted fetus
title_fullStr Isolation of equine herpesvirus-2 from the lung of an aborted fetus
title_full_unstemmed Isolation of equine herpesvirus-2 from the lung of an aborted fetus
title_sort isolation of equine herpesvirus-2 from the lung of an aborted fetus
publishDate 2005
url http://sedici.unlp.edu.ar/handle/10915/83372
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