Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup>
A high performance liquid chromatography photo diode array UV detection electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MS<sup>n</sup>) method was developed and validated for the quality evaluation of gamboge (dryed resin exuded from the stems of Garcinia hanburyi). The co...
Guardado en:
| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/8336 http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_14.pdf |
| Aporte de: |
| id |
I19-R120-10915-8336 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia gamboge; garcinia hanburyi; HPLC?PDA?ESI/MSn; quality control; traditional chinese medicine Plantas Medicinales Cromatografía Estudios de Evaluación |
| spellingShingle |
Farmacia gamboge; garcinia hanburyi; HPLC?PDA?ESI/MSn; quality control; traditional chinese medicine Plantas Medicinales Cromatografía Estudios de Evaluación Zhou, An Wu, Hongfei Peng, Daiyin Li, Qinglin Sun, Xiaojuan Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> |
| topic_facet |
Farmacia gamboge; garcinia hanburyi; HPLC?PDA?ESI/MSn; quality control; traditional chinese medicine Plantas Medicinales Cromatografía Estudios de Evaluación |
| description |
A high performance liquid chromatography photo diode array UV detection electrospray ionization tandem mass spectrometry (HPLC-PDA-ESI/MS<sup>n</sup>) method was developed and validated for the quality evaluation of gamboge (dryed resin exuded from the stems of Garcinia hanburyi). The contents of the three bioactive constituents (gambogenic acid, R-gambogic acid and S-gambogic) were determined by using HPLC–PDA, and their chemical structures were identified by HPLC-PDA-ESI/MS<sup>n</sup>.The limits of detection and quantitation were between 0.039-0.048 μg/mL and 0.13-0.16 μg/mL. The intra- and inter- assay precisions, in terms of percent relative standard deviation, are less than 3.7 and 4.8 %, respectively . The accuracy, in terms of recovery percentage, ranged from 96.86 to 101.70 %. Good linearity (correlation coefficient > 0.9996) for each calibration curve of standards. HPLC-PDA-ESI/MS<sup>n</sup> was use to analyze caged xanthones in gamboge. A total of 16 peaks were identified or tentatively characterized. The results indicated that the method could be considered to be a simple, rapid and reliable method for the quality evaluation of gamboge. |
| format |
Articulo Articulo |
| author |
Zhou, An Wu, Hongfei Peng, Daiyin Li, Qinglin Sun, Xiaojuan |
| author_facet |
Zhou, An Wu, Hongfei Peng, Daiyin Li, Qinglin Sun, Xiaojuan |
| author_sort |
Zhou, An |
| title |
Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> |
| title_short |
Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> |
| title_full |
Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> |
| title_fullStr |
Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> |
| title_full_unstemmed |
Qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by HPLC-PDA-ESI/MS<sup>n</sup> |
| title_sort |
qualitative and quantitative analyses of three bioactive compounds in traditional chinese medicine gamboge by hplc-pda-esi/ms<sup>n</sup> |
| publishDate |
2011 |
| url |
http://sedici.unlp.edu.ar/handle/10915/8336 http://www.latamjpharm.org/resumenes/30/8/LAJOP_30_8_1_14.pdf |
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Repositorios |
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1764820488538816516 |