Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial...
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Autores principales: | , , , , |
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Formato: | Articulo |
Lenguaje: | Inglés |
Publicado: |
2005
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Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83353 |
Aporte de: |
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I19-R120-10915-83353 |
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record_format |
dspace |
institution |
Universidad Nacional de La Plata |
institution_str |
I-19 |
repository_str |
R-120 |
collection |
SEDICI (UNLP) |
language |
Inglés |
topic |
Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution |
spellingShingle |
Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution Rumbo, Martín Courjault-Gautier, Françoise Sierro, Frédéric Sirard, Jean Claude Felley-Bosco, Emanuela Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
topic_facet |
Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution |
description |
Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. In particulate fractions, iNOS dimers were more abundant than in the cytosolic fraction. Similar observations were seen in mouse duodenum samples. These results suggest that, in epithelial cells, iNOS activity is regulated by localization-dependent processes. |
format |
Articulo Articulo |
author |
Rumbo, Martín Courjault-Gautier, Françoise Sierro, Frédéric Sirard, Jean Claude Felley-Bosco, Emanuela |
author_facet |
Rumbo, Martín Courjault-Gautier, Françoise Sierro, Frédéric Sirard, Jean Claude Felley-Bosco, Emanuela |
author_sort |
Rumbo, Martín |
title |
Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
title_short |
Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
title_full |
Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
title_fullStr |
Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
title_full_unstemmed |
Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
title_sort |
polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells |
publishDate |
2005 |
url |
http://sedici.unlp.edu.ar/handle/10915/83353 |
work_keys_str_mv |
AT rumbomartin polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT courjaultgautierfrancoise polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT sierrofrederic polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT sirardjeanclaude polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT felleyboscoemanuela polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells |
bdutipo_str |
Repositorios |
_version_ |
1764820488533573636 |