Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells

Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial...

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Detalles Bibliográficos
Autores principales:	Rumbo, Martín, Courjault-Gautier, Françoise, Sierro, Frédéric, Sirard, Jean Claude, Felley-Bosco, Emanuela
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2005
Materias:	Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/83353
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-83353
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution
spellingShingle	Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution Rumbo, Martín Courjault-Gautier, Françoise Sierro, Frédéric Sirard, Jean Claude Felley-Bosco, Emanuela Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
topic_facet	Biología Dimerization Inducible nitric oxide synthase Intestinal epithelial cells Specific activity Subcellular distribution
description	Inducible nitric oxide synthase (iNOS) functions as a homodimer. In cell extracts, iNOS molecules partition both in cytosolic and particulate fractions, indicating that iNOS exists as soluble and membrane associated forms. In this study, iNOS features were investigated in human intestinal epithelial cells stimulated with cytokines and in duodenum from mice exposed to flagellin. Our experiments indicate that iNOS is mainly associated with the particulate fraction of cell extracts. Confocal microscopy showed a preferential localization of iNOS at the apical pole of intestinal epithelial cells. In particulate fractions, iNOS dimers were more abundant than in the cytosolic fraction. Similar observations were seen in mouse duodenum samples. These results suggest that, in epithelial cells, iNOS activity is regulated by localization-dependent processes.
format	Articulo Articulo
author	Rumbo, Martín Courjault-Gautier, Françoise Sierro, Frédéric Sirard, Jean Claude Felley-Bosco, Emanuela
author_facet	Rumbo, Martín Courjault-Gautier, Françoise Sierro, Frédéric Sirard, Jean Claude Felley-Bosco, Emanuela
author_sort	Rumbo, Martín
title	Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
title_short	Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
title_full	Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
title_fullStr	Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
title_full_unstemmed	Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
title_sort	polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells
publishDate	2005
url	http://sedici.unlp.edu.ar/handle/10915/83353
work_keys_str_mv	AT rumbomartin polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT courjaultgautierfrancoise polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT sierrofrederic polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT sirardjeanclaude polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells AT felleyboscoemanuela polarizeddistributionofinduciblenitricoxidesynthaseregulatesactivityinintestinalepithelialcells
bdutipo_str	Repositorios
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Polarized distribution of inducible nitric oxide synthase regulates activity in intestinal epithelial cells

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