Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats

Objective: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity and <i>in vitro</i> retroperitoneal (RP) adipocyte function. Designs: A dose of 4 mg/g body weight (BW) of...

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Detalles Bibliográficos
Autores principales: Moreno, Griselda, Perelló, Mario, Camihort, Gisela, Luna, Georgina Cecilia, Cónsole-Avegliano, Gloria Miriam, Gaillard, R. C., Spinedi, Eduardo
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83294
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83294
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
spellingShingle Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
Moreno, Griselda
Perelló, Mario
Camihort, Gisela
Luna, Georgina Cecilia
Cónsole-Avegliano, Gloria Miriam
Gaillard, R. C.
Spinedi, Eduardo
Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
topic_facet Ciencias Médicas
Ciencias Exactas
ACTH
Fat morphometry
Glucocorticoid
Glucose
Insulin
Leptin
MSG
description Objective: To explore the effects of transient correction of enhanced corticoadrenal activity in monosodium L-glutamate (MSG)-damaged female rats on peripheral insulin sensitivity and <i>in vitro</i> retroperitoneal (RP) adipocyte function. Designs: A dose of 4 mg/g body weight (BW) of MSG or vehicle (CTR) was i.p. injected, once every 2 days, between days 2 and 10 of age, in female rats. Intact and 21 day-operated (sham or adrenal enucleation (AE)) rats from both (CTR and MSG) groups were used for experimentation on day 120 of age. Circulating levels of several hormones, in basal and after i.v. high-glucose load conditions, and RP adiposity morphology and function were then evaluated. Results: MSG rats developed increased adrenocortical function, hyperadiposity, hyperleptinemia, hyperinsulinemia and decreased peripheral insulin sensitivity. These characteristics were fully reversed after transient correction of corticoadrenal hyperactivity induced by AE. In addition, <i>in vitro</i> experimentation with isolated RP adipocytes indicated that cells from intact MSG animals displayed decreased sensitivity to insulin and dexamethasone stimulation of leptin secretion. Interestingly, adipocyte dysfunction in MSG rats was fully abrogated after AE-induced transient correction of insulinemia, leptinemia and adrenocortical activity. Importantly, the reversion of these metabolic abnormalities, induced by AE for 21 days, in MSG animals did occur, despite no significant changes in BW values. Conclusion: Our results support that the changes in adipocyte characteristics and peripheral insulin resistance, developed in this pseudo-obese female rat model, are mainly due to increased glucocorticoid production. Importantly, appropriate correction of the enhanced adrenocortical activity fully reversed these abnormal functions.
format Articulo
Articulo
author Moreno, Griselda
Perelló, Mario
Camihort, Gisela
Luna, Georgina Cecilia
Cónsole-Avegliano, Gloria Miriam
Gaillard, R. C.
Spinedi, Eduardo
author_facet Moreno, Griselda
Perelló, Mario
Camihort, Gisela
Luna, Georgina Cecilia
Cónsole-Avegliano, Gloria Miriam
Gaillard, R. C.
Spinedi, Eduardo
author_sort Moreno, Griselda
title Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_short Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_full Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_fullStr Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_full_unstemmed Impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
title_sort impact of transient correction of increased adrenocortical activity in hypothalamo-damaged, hyperadipose female rats
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83294
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