Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration

Administration of a sucrose-rich diet (SRD) to normal amsters induces an insulin-resistant state and a significant increase of insulin secretion and β-cell mass. Islets isolated from these animals had a marked increase in glucose metabolism and glucose-induced insulin secretion, at both low and high...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maiztegui, Bárbara, Borelli, María Inés, Massa, María Laura, Del Zotto, Héctor Herminio, Gagliardino, Juan José
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Médicas
sucrose
hexokinase
glucokinase
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83225
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83225
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Médicas
sucrose
hexokinase
glucokinase
spellingShingle Ciencias Médicas
sucrose
hexokinase
glucokinase
Maiztegui, Bárbara
Borelli, María Inés
Massa, María Laura
Del Zotto, Héctor Herminio
Gagliardino, Juan José
Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration
topic_facet Ciencias Médicas
sucrose
hexokinase
glucokinase
description Administration of a sucrose-rich diet (SRD) to normal amsters induces an insulin-resistant state and a significant increase of insulin secretion and β-cell mass. Islets isolated from these animals had a marked increase in glucose metabolism and glucose-induced insulin secretion, at both low and high glucose concentrations. They also presented increased hexokinase (HK) activity, without measurable changes in glucokinase (GK) activity. In this study we measured HK and GK activity in homogenates of islets isolated from normal control and SRD-fed hamsters, as well as in their particulate and cytosolic fractions. We also measured transcription rate (m-RNA by reverse transcriptase PCR) and expression levels (Western blotting) of both enzymes in these islets. We found an increase in HK activity and expression levels, without measurable changes in HK mRNA level in SRD-fed animals. Whereas a similar GK activity was measured in homogenates of islets isolated from both groups, such activity was significantly higher in the cytosolic fraction of SRD islets. On the other hand, GK transcription rate and expression level were similar in both experimental groups. Our results suggest that the increased β-cell secretory response to low glucose can be partly ascribed to an increased activity of islet HK consecutive to an enhanced expression of the enzyme, while the enhanced response to high glucose could be due to changes in GK compartmentalization.
format Articulo
Articulo
author Maiztegui, Bárbara
Borelli, María Inés
Massa, María Laura
Del Zotto, Héctor Herminio
Gagliardino, Juan José
author_facet Maiztegui, Bárbara
Borelli, María Inés
Massa, María Laura
Del Zotto, Héctor Herminio
Gagliardino, Juan José
author_sort Maiztegui, Bárbara
title Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration
title_short Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration
title_full Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration
title_fullStr Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration
title_full_unstemmed Enchanced expression of hexokinase I in pancreatic islets induced by sucrose administration
title_sort enchanced expression of hexokinase i in pancreatic islets induced by sucrose administration
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83225
work_keys_str_mv AT maizteguibarbara enchancedexpressionofhexokinaseiinpancreaticisletsinducedbysucroseadministration
AT borellimariaines enchancedexpressionofhexokinaseiinpancreaticisletsinducedbysucroseadministration
AT massamarialaura enchancedexpressionofhexokinaseiinpancreaticisletsinducedbysucroseadministration
AT delzottohectorherminio enchancedexpressionofhexokinaseiinpancreaticisletsinducedbysucroseadministration
AT gagliardinojuanjose enchancedexpressionofhexokinaseiinpancreaticisletsinducedbysucroseadministration
bdutipo_str Repositorios
_version_ 1764820488405647361

Ejemplares similares