High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study

A sensitive and accurate HPLC-UV method for the quantification of fluconazole (FNZ) in human plasma has been developed. The sample was prepared by liquid–liquid extraction (LLE) of FNZ from plasma using ethyl acetate. Nevirapine (NVP) was used as internal standard. The chromatographic retention time...

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Detalles Bibliográficos
Autores principales: Sousa, Carlos E.M., Bedor, Danilo C. G., Sampaio Fhilo, L. C. A., Silva, T. M., Bonifácio, F. N., Albuquerque, Miracy M., Santana, Davi P.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
bioequivalence; fluconazole; high performance liquid chromatography (HPLC); validation
Técnicas de Química Analítica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8318
http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_2_3.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8318
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
bioequivalence; fluconazole; high performance liquid chromatography (HPLC); validation
Técnicas de Química Analítica
spellingShingle Farmacia
bioequivalence; fluconazole; high performance liquid chromatography (HPLC); validation
Técnicas de Química Analítica
Sousa, Carlos E.M.
Bedor, Danilo C. G.
Sampaio Fhilo, L. C. A.
Silva, T. M.
Bonifácio, F. N.
Albuquerque, Miracy M.
Santana, Davi P.
High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
topic_facet Farmacia
bioequivalence; fluconazole; high performance liquid chromatography (HPLC); validation
Técnicas de Química Analítica
description A sensitive and accurate HPLC-UV method for the quantification of fluconazole (FNZ) in human plasma has been developed. The sample was prepared by liquid–liquid extraction (LLE) of FNZ from plasma using ethyl acetate. Nevirapine (NVP) was used as internal standard. The chromatographic retention times of FNZ and NVP were 3.4 and 5.7 min, respectively. The lower limit of quantitation (LLOQ) was 0.5 μg/mL, and no interferences were detected in the chromatograms. The HPLC-UV method was validated by evaluating its intra-day and inter-day precisions and accuracies in a linear concentration range between 0.5 and 8.0 μg/mL. The method was developed, validated and successfully applied to bioequivalence studies involving the oral administration of a single 150 mg FNZ capsules in healthy Brazilian male volunteers.
format Articulo
Comunicacion
author Sousa, Carlos E.M.
Bedor, Danilo C. G.
Sampaio Fhilo, L. C. A.
Silva, T. M.
Bonifácio, F. N.
Albuquerque, Miracy M.
Santana, Davi P.
author_facet Sousa, Carlos E.M.
Bedor, Danilo C. G.
Sampaio Fhilo, L. C. A.
Silva, T. M.
Bonifácio, F. N.
Albuquerque, Miracy M.
Santana, Davi P.
author_sort Sousa, Carlos E.M.
title High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
title_short High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
title_full High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
title_fullStr High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
title_full_unstemmed High-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
title_sort high-performance liquid chromatographic determination of fluconazole in plasma and its application to a bioequivalence study
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8318
http://www.latamjpharm.org/resumenes/30/7/LAJOP_30_7_2_3.pdf
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