Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations

In this study two new protocols with nonradioactive techniques applied on DNA analysis with RFLP (Restriction Fragment Length Polymorphism) were presented. They have been modified from the DIG (Boehringer Mannheim) and Photogene 2.0 (Life Technology) Systems. DNA probes were random-primed labeled wi...

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Detalles Bibliográficos
Autores principales:	Cordo, Cristina Alicia, Lojo, María de las Mercedes, Remorini, P.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2006
Materias:	Botánica Ciencias Agrarias DNA hybridization Nonradioactive technique RFLP Septoria tritici-Mycosphaerella graminicola
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/83169
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-83169
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Botánica Ciencias Agrarias DNA hybridization Nonradioactive technique RFLP Septoria tritici-Mycosphaerella graminicola
spellingShingle	Botánica Ciencias Agrarias DNA hybridization Nonradioactive technique RFLP Septoria tritici-Mycosphaerella graminicola Cordo, Cristina Alicia Lojo, María de las Mercedes Remorini, P. Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations
topic_facet	Botánica Ciencias Agrarias DNA hybridization Nonradioactive technique RFLP Septoria tritici-Mycosphaerella graminicola
description	In this study two new protocols with nonradioactive techniques applied on DNA analysis with RFLP (Restriction Fragment Length Polymorphism) were presented. They have been modified from the DIG (Boehringer Mannheim) and Photogene 2.0 (Life Technology) Systems. DNA probes were random-primed labeled with the specific compounds for each system. The quantity of digested DNA, the concentrations of the probe in the hybridization step and the concentration of the anti-body conjugate for the detection process were optimized. The improvements from the original protocol for the DIG System were an increase in the concentration of the labeled probe in the hybridization step and in the concentration of the anti-digoxigenin-AP conjugate in the detection process. There were no quantitative changes in the protocol of the Photogene System, but the quality of the washing process was improved for the post hybridization, blocking and detection steps. There is an economic advantage when using the Photogene System if the cost for reaction is analyzed. A brief comment about the frequency of the RFLP patterns from the isolates of both geographical sampled regions was also presented. The following advantages were found when these systems were used: safety in handling, no decay of labeled probes, reusable hybridization mixes (in the case of the DIG System), extremely short exposure times in the signal detection and economical differences in the cost per reaction for the Latin American economical conditions. This procedure saved time and cut costs without reducing sensitivity.
format	Articulo Articulo
author	Cordo, Cristina Alicia Lojo, María de las Mercedes Remorini, P.
author_facet	Cordo, Cristina Alicia Lojo, María de las Mercedes Remorini, P.
author_sort	Cordo, Cristina Alicia
title	Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations
title_short	Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations
title_full	Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations
title_fullStr	Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations
title_full_unstemmed	Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations
title_sort	two adapted techniques in studies of dna fingerprinting of septoria tritici populations
publishDate	2006
url	http://sedici.unlp.edu.ar/handle/10915/83169
work_keys_str_mv	AT cordocristinaalicia twoadaptedtechniquesinstudiesofdnafingerprintingofseptoriatriticipopulations AT lojomariadelasmercedes twoadaptedtechniquesinstudiesofdnafingerprintingofseptoriatriticipopulations AT remorinip twoadaptedtechniquesinstudiesofdnafingerprintingofseptoriatriticipopulations
bdutipo_str	Repositorios
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Two adapted techniques in studies of DNA fingerprinting of Septoria tritici populations

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