Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis
The aim of the present study was to evaluate inhibin secretion in rats with autoimmune orchitis. As we have previously described, experimental autoimmune orchitis (EAO) induced in rats by active immunization with testis homogenate and adjuvants is characterized by an interstitial mononuclear cell in...
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| Autores principales: | , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
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2001
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83167 |
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I19-R120-10915-83167 |
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dspace |
| institution |
Universidad Nacional de La Plata |
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I-19 |
| repository_str |
R-120 |
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SEDICI (UNLP) |
| language |
Inglés |
| topic |
Ciencias Exactas inhibin secretion damage of seminiferous tubules rats |
| spellingShingle |
Ciencias Exactas inhibin secretion damage of seminiferous tubules rats Suescun, María Olga Lustig, Livia Calandra, Ricardo Saúl Groome, Nigel P. Campo, Silvia Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| topic_facet |
Ciencias Exactas inhibin secretion damage of seminiferous tubules rats |
| description |
The aim of the present study was to evaluate inhibin secretion in rats with autoimmune orchitis. As we have previously described, experimental autoimmune orchitis (EAO) induced in rats by active immunization with testis homogenate and adjuvants is characterized by an interstitial mononuclear cell infiltrate and sloughing of the germinal epithelium. At 120 days after the first immunization 60% of the rats exhibited a severe orchitis with large areas of aspermatogenic seminiferous tubules in which only spermatogonia and Sertoli cells with cytoplasmic vacuolization remained attached to the tubular wall. None of the untreated (N) or control (C) rats revealed pathological alterations. Sixty percent decrease in testis weight was observed in rats with EAO compared with N or C groups. A 3-fold increase in serum FSH levels was observed in rats with EAO compared with N or C groups (19·8 ± 3·7 vs 5·6 ± 0·3 and 5·9 ± 0·1 ng/ml respectively). A significant decrease in inhibin B levels was observed in rats with EAO when compared with N or C groups (40 ± 4·6 vs 207 ± 38·8 and 221·4 ± 28·6 pg/ml respectively). An inverse correlation between inhibin B and FSH serum levels and a direct correlation between inhibin B and testis weight were found. Strong expression of the inhibin α-subunit in Sertoli cells of untreated and control rats was observed; this subunit was undetectable or poorly detectable in rats with orchitis. Positive staining for the inhibin α-subunit was also observed in Leydig cells of all groups studied. In conclusion, using a model of autoimmune orchitis our results show that circulating inhibin B levels and inhibin α-subunit expression in Sertoli cell cytoplasm closely correlate with the degree of damage of the germinal epithelium. |
| format |
Articulo Articulo |
| author |
Suescun, María Olga Lustig, Livia Calandra, Ricardo Saúl Groome, Nigel P. Campo, Silvia |
| author_facet |
Suescun, María Olga Lustig, Livia Calandra, Ricardo Saúl Groome, Nigel P. Campo, Silvia |
| author_sort |
Suescun, María Olga |
| title |
Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| title_short |
Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| title_full |
Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| title_fullStr |
Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| title_full_unstemmed |
Correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| title_sort |
correlation between inhibin secretion and damage of seminiferous tubules in a model of experimental autoimmune orchitis |
| publishDate |
2001 |
| url |
http://sedici.unlp.edu.ar/handle/10915/83167 |
| work_keys_str_mv |
AT suescunmariaolga correlationbetweeninhibinsecretionanddamageofseminiferoustubulesinamodelofexperimentalautoimmuneorchitis AT lustiglivia correlationbetweeninhibinsecretionanddamageofseminiferoustubulesinamodelofexperimentalautoimmuneorchitis AT calandraricardosaul correlationbetweeninhibinsecretionanddamageofseminiferoustubulesinamodelofexperimentalautoimmuneorchitis AT groomenigelp correlationbetweeninhibinsecretionanddamageofseminiferoustubulesinamodelofexperimentalautoimmuneorchitis AT camposilvia correlationbetweeninhibinsecretionanddamageofseminiferoustubulesinamodelofexperimentalautoimmuneorchitis |
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Repositorios |
| _version_ |
1764820488356364288 |