Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation

Previous reports showed the protective effect of the synthetic antioxidant butylated hydroxytoluene (BHT) against the chromosomal damage induced by bleomycin (BLM), cadmium chloride and potassium dichromate. To test the hypothesis that this effect was exerted by inhibition and/or scavenging of react...

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Detalles Bibliográficos
Autores principales: Grillo, Claudia Alejandra, Dulout, Fernando Noel
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Veterinarias
butylated hydroxytoluene
chromosomal damage
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/83133
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-83133
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Veterinarias
butylated hydroxytoluene
chromosomal damage
spellingShingle Ciencias Veterinarias
butylated hydroxytoluene
chromosomal damage
Grillo, Claudia Alejandra
Dulout, Fernando Noel
Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation
topic_facet Ciencias Veterinarias
butylated hydroxytoluene
chromosomal damage
description Previous reports showed the protective effect of the synthetic antioxidant butylated hydroxytoluene (BHT) against the chromosomal damage induced by bleomycin (BLM), cadmium chloride and potassium dichromate. To test the hypothesis that this effect was exerted by inhibition and/or scavenging of reactive oxygen species (ROS), the effect of BHT on the chromosomal damage induced by a high dose-rate gamma rays (HDR <SUP>192</SUP>Ir). Experiments were carried out by irradiating G<SUB>1</SUB> CHO cells with nominal doses of 1, 2 or 3 Gy. BHT (doses of 1.0, 2.5 or 5.0 μg/ml) was added to the culture immediately before or immediately after irradiation. Cells were then incubated in the presence of BHT for 13 h until harvesting and fixation. Results obtained showed that BHT did not decrease the chromosomal damage induced by radiation in any consistent fashion. On the contrary, in cells post-treated with 5.0 μg/ml of BHT the yield of chromosomal aberrations increased in several experimental points. These results with ionizing radiation suggest that the previous observed protective effects of BHT on the chromosomal damage induced by chemical genotoxicants may not be mediated solely through the scavenging or inactivating reactive oxidative species. The decrease of the yield of chromosomal damage induced by BLM could be due to the union of BHT with a metallic ion, in this case Fe (II), required for the activation of BLM. In the same way, the protective effect of BHT on the chromosomal damage induced by cadmium chloride and potassium dichromate could be due to the decrease of the effective dose of both salts in the cell through the chelation of the cations by BHT.
format Articulo
Articulo
author Grillo, Claudia Alejandra
Dulout, Fernando Noel
author_facet Grillo, Claudia Alejandra
Dulout, Fernando Noel
author_sort Grillo, Claudia Alejandra
title Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation
title_short Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation
title_full Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation
title_fullStr Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation
title_full_unstemmed Butylated hydroxytoluene does not protect Chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192Ir irradiation
title_sort butylated hydroxytoluene does not protect chinese hamster ovary cells from chromosomal damage induced by high-dose rate 192ir irradiation
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/83133
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AT duloutfernandonoel butylatedhydroxytoluenedoesnotprotectchinesehamsterovarycellsfromchromosomaldamageinducedbyhighdoserate192irirradiation
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