Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia
Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity...
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| Autores principales: | , , , , , , , , , , , , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2007
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/83078 |
| Aporte de: |
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I19-R120-10915-83078 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Bioquímica Sinorhizobium meliloti Medicago sativa nodH gene |
| spellingShingle |
Bioquímica Sinorhizobium meliloti Medicago sativa nodH gene Del Papa, María Florencia Pistorio, Mariano Draghi, Walter Omar Lozano, Mauricio Javier Giusti, María de los Ángeles Medina, C. Van Dillewijn, P. Martínez-Abarca, F. Flores, B.M. Ruiz-Sainz, J.E. Megías, M. Pühler, A. Niehaus, K. Toro, N. Lagares, Antonio Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
| topic_facet |
Bioquímica Sinorhizobium meliloti Medicago sativa nodH gene |
| description |
Nodulation of Medicago sativa (alfalfa) is known to be restricted to Sinorhizobium meliloti and a few other rhizobia that include the poorly characterized isolates related to Rhizobium sp. strain Or191. Distinctive features of the symbiosis between alfalfa and S. meliloti are the marked specificity from the plant to the bacteria and the strict requirement for the presence of sulfated lipochitooligosaccharides (Nod factors [NFs]) at its reducing end. Here, we present evidence of the presence of a functional nodH-encoded NF sulfotransferase in the Or191-like rhizobia. The nodH gene, present in single copy, maps to a high molecular weight megaplasmid. As in S. meliloti, a nodF homolog was identified immediately upstream of nodH that was transcribed in the opposite direction (local synteny). This novel nodH ortholog was cloned and shown to restore both NF sulfation and the Nif +Fix+ phenotypes when introduced into an S. meliloti nodH mutant. Unexpectedly, however, nodH disruption in the Or191-like bacteria did not abolish their ability to nodulate alfalfa, resulting instead in a severely delayed nodulation. In agreement with evidence from other authors, the nodH sequence analysis strongly supports the idea that the Or191-like rhizobia most likely represent a genetic mosaic resulting from the horizontal transfer of symbiotic genes from a sinorhizobial megaplasmid to a not yet clearly identified ancestor. |
| format |
Articulo Articulo |
| author |
Del Papa, María Florencia Pistorio, Mariano Draghi, Walter Omar Lozano, Mauricio Javier Giusti, María de los Ángeles Medina, C. Van Dillewijn, P. Martínez-Abarca, F. Flores, B.M. Ruiz-Sainz, J.E. Megías, M. Pühler, A. Niehaus, K. Toro, N. Lagares, Antonio |
| author_facet |
Del Papa, María Florencia Pistorio, Mariano Draghi, Walter Omar Lozano, Mauricio Javier Giusti, María de los Ángeles Medina, C. Van Dillewijn, P. Martínez-Abarca, F. Flores, B.M. Ruiz-Sainz, J.E. Megías, M. Pühler, A. Niehaus, K. Toro, N. Lagares, Antonio |
| author_sort |
Del Papa, María Florencia |
| title |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
| title_short |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
| title_full |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
| title_fullStr |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
| title_full_unstemmed |
Identification and characterization of a nodH ortholog from the alfalfa-nodulating Or191-like rhizobia |
| title_sort |
identification and characterization of a nodh ortholog from the alfalfa-nodulating or191-like rhizobia |
| publishDate |
2007 |
| url |
http://sedici.unlp.edu.ar/handle/10915/83078 |
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