Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves

The effects of growth irradiance and respiration on ascorbic acid (AA) synthesis and accumulation were studied in the leaves of wild-type and transformed Arabidopsis thaliana with modified amounts of the mitochondrial alternative oxidase (AOX) protein. Plants were grown under low (LL; 50 μmol photon...

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Detalles Bibliográficos
Autores principales: Bartoli, Carlos Guillermo, Yu, Jianping, Gomez, Facundo Martin, Fernández, Laura Virginia, McIntosh, Lee, Foyer, Christine H.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2006
Materias:
Ciencias Naturales
Ciencias Agrarias
Alternative oxidase
Ascorbic acid
Cytochrome c
Light acclimation
Mitochondria
Respiration
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82988
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82988
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Naturales
Ciencias Agrarias
Alternative oxidase
Ascorbic acid
Cytochrome c
Light acclimation
Mitochondria
Respiration
spellingShingle Ciencias Naturales
Ciencias Agrarias
Alternative oxidase
Ascorbic acid
Cytochrome c
Light acclimation
Mitochondria
Respiration
Bartoli, Carlos Guillermo
Yu, Jianping
Gomez, Facundo Martin
Fernández, Laura Virginia
McIntosh, Lee
Foyer, Christine H.
Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
topic_facet Ciencias Naturales
Ciencias Agrarias
Alternative oxidase
Ascorbic acid
Cytochrome c
Light acclimation
Mitochondria
Respiration
description The effects of growth irradiance and respiration on ascorbic acid (AA) synthesis and accumulation were studied in the leaves of wild-type and transformed Arabidopsis thaliana with modified amounts of the mitochondrial alternative oxidase (AOX) protein. Plants were grown under low (LL; 50 μmol photons m<SUP>-2</SUP> s<SUP>-1</SUP>), intermediate (IL; 100 μmol photons m<SUP>-2</SUP> s<SUP>-1</SUP>), or high (HL; 250 μmol photons m<SUP>-2</SUP> s<SUP>-1</SUP>) light. Increasing growth irradiance progressively elevated leaf AA content and hence the values of dark-induced disappearance of leaf AA, which were 11, 55, and 89 nmol AA lost g<SUP>-1</SUP> fresh weight h<SUP>-1</SUP>, from LL-, IL-, and HL-grown leaves, respectively. When HL leaves were supplied with L-galactone-1,4-lactone (L-GalL; the precursor of AA), they accumulated twice as much AA and had double the maximal L-galactone-1,4-lactone dehydrogenase (L-GalLDH) activities of LL leaves. Growth under HL enhanced dehydroascorbate reductase and monodehydroascorbate reductase activities. Leaf respiration rates were highest in the HL leaves, which also had higher amounts of cytochrome c and cytochrome c oxidase (CCO) activities, as well as enhanced capacity of the AOX and CCO electron transport pathways. Leaves of the AOX-overexpressing lines accumulated more AA than wild-type or antisense leaves, particularly at HL. Intact mitochondria from AOX-overexpressing lines had higher AA synthesis capacities than those from the wild-type or antisense lines even though they had similar L-GalLDH activities. AOX antisense lines had more cytochrome c protein than wild-type or AOX-overexpressing lines. It is concluded that regardless of limitations on L-GalL synthesis by regulation of early steps in the AA synthesis pathway, the regulation of L-GalLDH activity via the interaction of light and respiratory controls is a crucial determinant of the overall ability of leaves to produce and accumulate AA.
format Articulo
Articulo
author Bartoli, Carlos Guillermo
Yu, Jianping
Gomez, Facundo Martin
Fernández, Laura Virginia
McIntosh, Lee
Foyer, Christine H.
author_facet Bartoli, Carlos Guillermo
Yu, Jianping
Gomez, Facundo Martin
Fernández, Laura Virginia
McIntosh, Lee
Foyer, Christine H.
author_sort Bartoli, Carlos Guillermo
title Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
title_short Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
title_full Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
title_fullStr Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
title_full_unstemmed Inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in Arabidopsis thaliana leaves
title_sort inter-relationships between light and respiration in the control of ascorbic acid synthesis and accumulation in arabidopsis thaliana leaves
publishDate 2006
url http://sedici.unlp.edu.ar/handle/10915/82988
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