The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis

Despite excellent vaccine coverage in developed countries, whooping cough is a reemerging disease that can be caused by two closely related pathogens, Bordetella pertussis and B. parapertussis. The two are antigenically distinct, and current vaccines, containing only B. pertussis-derived antigens, c...

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Autores principales: Zhang, Xuqing, Goebel, Elizabeth M., Rodríguez, María Eugenia, Preston, Andrew, Harvill, Eric T.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Química
Antigen
Bordetella parapertussis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82771
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82771
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Química
Antigen
Bordetella parapertussis
spellingShingle Química
Antigen
Bordetella parapertussis
Zhang, Xuqing
Goebel, Elizabeth M.
Rodríguez, María Eugenia
Preston, Andrew
Harvill, Eric T.
The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
topic_facet Química
Antigen
Bordetella parapertussis
description Despite excellent vaccine coverage in developed countries, whooping cough is a reemerging disease that can be caused by two closely related pathogens, Bordetella pertussis and B. parapertussis. The two are antigenically distinct, and current vaccines, containing only B. pertussis-derived antigens, confer efficient protection against B. pertussis but not against B. parapertussis. B. pertussis does not express the O antigen, while B. parapertussis retains it as a dominant surface antigen. Since the O antigen is a protective antigen for many pathogenic bacteria, we examined whether this factor is a potential protective antigen for B. parapertussis. In a mouse model of infection, immunization with wild-type B. parapertussis elicited a strong antibody response to the O antigen and conferred efficient protection against a subsequent B. parapertussis challenge. However, immunization with an isogenic mutant lacking the O antigen, B. parapertussis Δwbm, induced antibodies that recognized other antigens but did not efficiently mediate opsonophagocytosis of B. parapertussis. The passive transfer of sera raised against B. parapertussis, but not B. parapertussis Δwbm, reduced B. parapertussis loads in the lower respiratory tracts of mice. The addition of 10 μg of purified B. parapertussis lipopolysaccharide (LPS), which contains the O antigen, but not B. parapertussis Δwbm LPS drastically improved the efficacy of the acellular vaccine Adacel against B. parapertussis. These data suggest that the O antigen is a critical protective antigen of B. parapertussis and its inclusion can substantially improve whooping cough vaccine efficacy against this pathogen.
format Articulo
Articulo
author Zhang, Xuqing
Goebel, Elizabeth M.
Rodríguez, María Eugenia
Preston, Andrew
Harvill, Eric T.
author_facet Zhang, Xuqing
Goebel, Elizabeth M.
Rodríguez, María Eugenia
Preston, Andrew
Harvill, Eric T.
author_sort Zhang, Xuqing
title The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
title_short The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
title_full The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
title_fullStr The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
title_full_unstemmed The O antigen is a critical antigen for the development of a protective immune response to Bordetella parapertussis
title_sort o antigen is a critical antigen for the development of a protective immune response to bordetella parapertussis
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/82771
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