Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS

A simple and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was established and validated for the determination of prodrug of ginkgolide B (PGB) and its metabolite, ginkgolide B (GB) in rat plasma. The separation was achieved on a Waters Symmetry Shield RP18 column (150 m...

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Autores principales: Pan, Jian, Wu, Zeyu, Yuan, Yuan, Hui, Ailing, Yang, Yi
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Prodrogas
ginkgolide B; LC-MS/MS; pharmacokinetics; plasma; prodrug of ginkgolide B
Terpenos
Cromatografía Liquida
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8276
http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_1_18.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8276
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Prodrogas
ginkgolide B; LC-MS/MS; pharmacokinetics; plasma; prodrug of ginkgolide B
Terpenos
Cromatografía Liquida
spellingShingle Farmacia
Prodrogas
ginkgolide B; LC-MS/MS; pharmacokinetics; plasma; prodrug of ginkgolide B
Terpenos
Cromatografía Liquida
Pan, Jian
Wu, Zeyu
Yuan, Yuan
Hui, Ailing
Yang, Yi
Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS
topic_facet Farmacia
Prodrogas
ginkgolide B; LC-MS/MS; pharmacokinetics; plasma; prodrug of ginkgolide B
Terpenos
Cromatografía Liquida
description A simple and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method was established and validated for the determination of prodrug of ginkgolide B (PGB) and its metabolite, ginkgolide B (GB) in rat plasma. The separation was achieved on a Waters Symmetry Shield RP18 column (150 mm × 3.9 mm i.d., 5 μm particle size), using a mobile phase composed of methanol/water with 10 mM ammonium acetate (85:15, v/v) at a flow rate of 800 μL/min in 2 min. An API 3200 triple quadrupole mass spectrometer equipped with electrospray ionization source was operated in negative ionization mode. Multiple reaction monitoring (MRM) was performed to quantify PGB, GB and the internal standard (IS) at m/z transitions of 528.1 → 122.0, 423.4 → 367.3, 407.5 → 351.2, respectively. A good linearity was found. Intra- and inter-day precision, accuracy, extraction recovery, matrix effect and stability were validated to be within an acceptable range. The developed method was successfully applied to a pharmacokinetic study after intravenous administration of a 10 mg/kg dose of PGB to rats.
format Articulo
Articulo
author Pan, Jian
Wu, Zeyu
Yuan, Yuan
Hui, Ailing
Yang, Yi
author_facet Pan, Jian
Wu, Zeyu
Yuan, Yuan
Hui, Ailing
Yang, Yi
author_sort Pan, Jian
title Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS
title_short Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS
title_full Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS
title_fullStr Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS
title_full_unstemmed Simultaneous determination of prodrug of Ginkgolide B and Ginkgolide B in rat plasma by LC-MS/MS
title_sort simultaneous determination of prodrug of ginkgolide b and ginkgolide b in rat plasma by lc-ms/ms
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8276
http://www.latamjpharm.org/resumenes/30/6/LAJOP_30_6_1_18.pdf
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AT wuzeyu simultaneousdeterminationofprodrugofginkgolidebandginkgolidebinratplasmabylcmsms
AT yuanyuan simultaneousdeterminationofprodrugofginkgolidebandginkgolidebinratplasmabylcmsms
AT huiailing simultaneousdeterminationofprodrugofginkgolidebandginkgolidebinratplasmabylcmsms
AT yangyi simultaneousdeterminationofprodrugofginkgolidebandginkgolidebinratplasmabylcmsms
bdutipo_str Repositorios
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