Mucosal tissue transglutaminase expression in celiac disease

Tissue transglutaminase (tTG) plays an important role in celiac disease pathogenesis and antibodies to tTG are a diagnostic marker of gluten-sensitive enteropathy. The aim of this study was to investigate the localization of tTG in the duodenal mucosa in control tissues and in different histological...

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Autores principales:	Villanacci, Vincenzo, Not, Tarcisio, Sblattero, Daniele, Gaiotto, Tiziano, Chirdo, Fernando Gabriel, Galletti, Anna, Bassotti, Gabrio
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2009
Materias:	Ciencias Médicas Celiac disease Duodenal mucosa Immunohistochemistry Monoclonal antibodies Sprue Tissue transglutaminase
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82683
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82683
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Ciencias Médicas Celiac disease Duodenal mucosa Immunohistochemistry Monoclonal antibodies Sprue Tissue transglutaminase
spellingShingle	Ciencias Médicas Celiac disease Duodenal mucosa Immunohistochemistry Monoclonal antibodies Sprue Tissue transglutaminase Villanacci, Vincenzo Not, Tarcisio Sblattero, Daniele Gaiotto, Tiziano Chirdo, Fernando Gabriel Galletti, Anna Bassotti, Gabrio Mucosal tissue transglutaminase expression in celiac disease
topic_facet	Ciencias Médicas Celiac disease Duodenal mucosa Immunohistochemistry Monoclonal antibodies Sprue Tissue transglutaminase
description	Tissue transglutaminase (tTG) plays an important role in celiac disease pathogenesis and antibodies to tTG are a diagnostic marker of gluten-sensitive enteropathy. The aim of this study was to investigate the localization of tTG in the duodenal mucosa in control tissues and in different histological stages of celiac disease by using a commercial and a novel set of anti-tTG monoclonal antibodies, to see whether this assessment can be useful for diagnostic purpose. The distribution of tTG was firstly evaluated in 18 untreated celiac patients by using a commercial monoclonal antibody (CUB7402) against tissue transglutaminase enzyme and directed against the loop-core region of the enzyme. Thereafter, in further 30 untreated celiac patients we employed three newly characterized anti-tTG monoclonal antibodies produced against recombinant human-tTG. The epitopes recognized are located in three distinct domains of the protein corresponding to the core, C1 and C2 protein structure. Eleven age- and sex-matched patients with chronic duodenitis acted as controls. All subjects underwent upper endoscopy to obtain biopsy samples from the duodenum. Overall, we found that (i) tTG is equally expressed in CD at different stages of disease; (ii) tTG is expressed, at similar level, in CD and controls with duodenitis. Assessment of tTG level in biopsy samples by immunohistochemical methods is not useful in the clinical diagnostic work-up of CD.
format	Articulo Articulo
author	Villanacci, Vincenzo Not, Tarcisio Sblattero, Daniele Gaiotto, Tiziano Chirdo, Fernando Gabriel Galletti, Anna Bassotti, Gabrio
author_facet	Villanacci, Vincenzo Not, Tarcisio Sblattero, Daniele Gaiotto, Tiziano Chirdo, Fernando Gabriel Galletti, Anna Bassotti, Gabrio
author_sort	Villanacci, Vincenzo
title	Mucosal tissue transglutaminase expression in celiac disease
title_short	Mucosal tissue transglutaminase expression in celiac disease
title_full	Mucosal tissue transglutaminase expression in celiac disease
title_fullStr	Mucosal tissue transglutaminase expression in celiac disease
title_full_unstemmed	Mucosal tissue transglutaminase expression in celiac disease
title_sort	mucosal tissue transglutaminase expression in celiac disease
publishDate	2009
url	http://sedici.unlp.edu.ar/handle/10915/82683
work_keys_str_mv	AT villanaccivincenzo mucosaltissuetransglutaminaseexpressioninceliacdisease AT nottarcisio mucosaltissuetransglutaminaseexpressioninceliacdisease AT sblatterodaniele mucosaltissuetransglutaminaseexpressioninceliacdisease AT gaiottotiziano mucosaltissuetransglutaminaseexpressioninceliacdisease AT chirdofernandogabriel mucosaltissuetransglutaminaseexpressioninceliacdisease AT gallettianna mucosaltissuetransglutaminaseexpressioninceliacdisease AT bassottigabrio mucosaltissuetransglutaminaseexpressioninceliacdisease
bdutipo_str	Repositorios
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Mucosal tissue transglutaminase expression in celiac disease

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