Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle

Aims: To isolate, characterize and select phages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing Escherichia coli (EHEC and STEC) in cattle. Methods and Results: Sixteen STEC and EHEC coliphages were isolated from bovine minced meat and stool samples and characterized w...

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Detalles Bibliográficos
Autores principales:	Dini, Cecilia, Urraza, Patricio José de
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2010
Materias:	Química bacteriophage(s) biocontrol E. coli (all potentially pathogenic types) food safety acid
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/82531
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-82531
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Química bacteriophage(s) biocontrol E. coli (all potentially pathogenic types) food safety acid
spellingShingle	Química bacteriophage(s) biocontrol E. coli (all potentially pathogenic types) food safety acid Dini, Cecilia Urraza, Patricio José de Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle
topic_facet	Química bacteriophage(s) biocontrol E. coli (all potentially pathogenic types) food safety acid
description	Aims: To isolate, characterize and select phages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing Escherichia coli (EHEC and STEC) in cattle. Methods and Results: Sixteen STEC and EHEC coliphages were isolated from bovine minced meat and stool samples and characterized with respect to their host range against STEC, EHEC and other Gram-negative pathogens; their morphology by electron microscopy; the presence of the stx1, stx2 and cI genes by means of PCR; RAPD and rep-PCR profiles; plaque formation; and acid resistance. Six isolates belonged to the Myoviridae and 10 to the Podoviridae families. The phages negative for stx and cI that formed large, well-defined plaques were all isolated using EHEC O157:H7 as host. Among them, only CA911 was a myophage and, together with CA933P, had the broadest host range for STEC and EHEC; the latter phage also infected Shigella and Pseudomonas. Isolates CA911, MFA933P and MFA45D differed in particle morphology and amplification patterns by RAPD and rep-PCR and showed the highest acidity tolerance. Conclusions: Myophage CA911 and podophages CA933P, MFA933P and MFA45D were chosen as the best candidates for biocontrol of STEC and EHEC in cattle. Significance and Impact of the Study: This work employs steps for a rational selection and characterization of bacteriophages as therapeutic agents. This report constitutes the first documentation of STEC and EHEC phages isolated in Argentina and proposes for the first time the use of rep-PCR as a complement of RAPD on DNA fingerprinting of phages.
format	Articulo Articulo
author	Dini, Cecilia Urraza, Patricio José de
author_facet	Dini, Cecilia Urraza, Patricio José de
author_sort	Dini, Cecilia
title	Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle
title_short	Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle
title_full	Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle
title_fullStr	Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle
title_full_unstemmed	Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle
title_sort	isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and shiga toxin-producing e. coli(ehec and stec) in cattle
publishDate	2010
url	http://sedici.unlp.edu.ar/handle/10915/82531
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bdutipo_str	Repositorios
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Isolation and selection of coliphages as potential biocontrol agents of enterohemorrhagic and Shiga toxin-producing E. coli(EHEC and STEC) in cattle

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