Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses

High-throughput technology has facilitated genome-scale analyses of transcriptomic adjustments in response to environmental perturbations with an oxygen deprivation component, such as transient hypoxia or anoxia, root waterlogging, or complete submergence. We showed previously that Arabidopsis (Arab...

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Detalles Bibliográficos
Autores principales: Mustroph, Angelika, Lee, Seung Cho, Oosumi, Teruko, Zanetti, María Eugenia, Yang, Huijun, Ma, Kelvin, Yaghoubi-Masihi, Arbi, Fukao, Takeshi, Bailey-Serres, Julia
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Biología
oxygen
anoxia
Arabidopsis
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/82520
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-82520
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Biología
oxygen
anoxia
Arabidopsis
spellingShingle Biología
oxygen
anoxia
Arabidopsis
Mustroph, Angelika
Lee, Seung Cho
Oosumi, Teruko
Zanetti, María Eugenia
Yang, Huijun
Ma, Kelvin
Yaghoubi-Masihi, Arbi
Fukao, Takeshi
Bailey-Serres, Julia
Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
topic_facet Biología
oxygen
anoxia
Arabidopsis
description High-throughput technology has facilitated genome-scale analyses of transcriptomic adjustments in response to environmental perturbations with an oxygen deprivation component, such as transient hypoxia or anoxia, root waterlogging, or complete submergence. We showed previously that Arabidopsis (Arabidopsis thaliana) seedlings elevate the levels of hundreds of transcripts, including a core group of 49 genes that are prioritized for translation across cell types of both shoots and roots. To recognize low-oxygen responses that are evolutionarily conserved versus species specific, we compared the transcriptomic reconfiguration in 21 organisms from four kingdoms (Plantae, Animalia, Fungi, and Bacteria). Sorting of organism proteomes into clusters of putative orthologs identified broadly conserved responses associated with glycolysis, fermentation, alternative respiration, metabolite transport, reactive oxygen species amelioration, chaperone activity, and ribosome biogenesis. Differ-entially regulated genes involved in signaling and transcriptional regulation were poorly conserved across kingdoms. Strikingly, nearly half of the induced mRNAs of Arabidopsis seedlings encode proteins of unknown function, of which over 40% had up-regulated orthologs in poplar (Populus trichocarpa), rice (Oryza sativa), or Chlamydomonas reinhardtii. Sixteen HYPOXIA-RESPONSIVE UNKNOWN PROTEIN (HUP) genes, including four that are Arabidopsis specific, were ectopically overexpressed and evaluated for their effect on seedling tolerance to oxygen deprivation. This allowed the identification of HUPs coregulated with genes associated with anaerobic metabolism and other processes that significantly enhance or reduce stress survival when ectopically overexpressed. These findings illuminate both broadly conserved and plant-specific low-oxygen stress responses and confirm that plant-specific HUPs with limited phylogenetic distribution influence low-oxygen stress endurance.
format Articulo
Articulo
author Mustroph, Angelika
Lee, Seung Cho
Oosumi, Teruko
Zanetti, María Eugenia
Yang, Huijun
Ma, Kelvin
Yaghoubi-Masihi, Arbi
Fukao, Takeshi
Bailey-Serres, Julia
author_facet Mustroph, Angelika
Lee, Seung Cho
Oosumi, Teruko
Zanetti, María Eugenia
Yang, Huijun
Ma, Kelvin
Yaghoubi-Masihi, Arbi
Fukao, Takeshi
Bailey-Serres, Julia
author_sort Mustroph, Angelika
title Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
title_short Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
title_full Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
title_fullStr Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
title_full_unstemmed Cross-Kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
title_sort cross-kingdom comparison of transcriptomic adjustments to low-oxygen stress highlights conserved and plant-specific responses
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/82520
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