Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms

In the present study, a simple, rapid and precise liquid chromatographic method was developed and validated for the determination of ezetimibe in its dosage form. Ezetimibe was separated in a 100 x 4.6 mm i.d., C18 column, 3 um particle size, Luna phenomenex, using a mobile phase composition of wate...

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Detalles Bibliográficos
Autores principales:	Nakarani, Mahendra P., Patel, Priyal R., Patel, Jayavdan K., Vaghani, Subhash S.
Formato:	Articulo
Lenguaje:	Español
Publicado:	2011
Materias:	Farmacia Cromatografía Líquida de Alta Presión medicamento ezetimibe; RP-HPLC; self emulsifying drug delivery Formas de Dosificación Placebos Colesterol
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8233 http://www.latamjpharm.org/resumenes/30/5/LAJOP_30_5_1_9.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Español
topic	Farmacia Cromatografía Líquida de Alta Presión medicamento ezetimibe; RP-HPLC; self emulsifying drug delivery Formas de Dosificación Placebos Colesterol
spellingShingle	Farmacia Cromatografía Líquida de Alta Presión medicamento ezetimibe; RP-HPLC; self emulsifying drug delivery Formas de Dosificación Placebos Colesterol Nakarani, Mahendra P. Patel, Priyal R. Patel, Jayavdan K. Vaghani, Subhash S. Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
topic_facet	Farmacia Cromatografía Líquida de Alta Presión medicamento ezetimibe; RP-HPLC; self emulsifying drug delivery Formas de Dosificación Placebos Colesterol
description	In the present study, a simple, rapid and precise liquid chromatographic method was developed and validated for the determination of ezetimibe in its dosage form. Ezetimibe was separated in a 100 x 4.6 mm i.d., C18 column, 3 um particle size, Luna phenomenex, using a mobile phase composition of water and acetonitrile (60:40 v/v). Column oven temperature was kept at 25 °C. The flow rate was 1.5 mL/min and the analyte monitored at 225 nm. The retention time of Ezetimibe was 8.47 min. The specificity of the method was determined by assessing interference from the placebo and by stress testing of the drug (forced degradation).The developed method was validated in terms of linearity, accuracy, precision, system suitability, limit of detection, limit of quantitation and solution stability. The proposed method was also applied successfully to the pharmaceutical dosage form self emulsified drug delivery without any interference by excipients.
format	Articulo Articulo
author	Nakarani, Mahendra P. Patel, Priyal R. Patel, Jayavdan K. Vaghani, Subhash S.
author_facet	Nakarani, Mahendra P. Patel, Priyal R. Patel, Jayavdan K. Vaghani, Subhash S.
author_sort	Nakarani, Mahendra P.
title	Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
title_short	Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
title_full	Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
title_fullStr	Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
title_full_unstemmed	Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms
title_sort	development and validation of stability indicating rp-hplc method for the determination of ezetimibe in pharmaceutical dosage forms
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8233 http://www.latamjpharm.org/resumenes/30/5/LAJOP_30_5_1_9.pdf
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bdutipo_str	Repositorios
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Development and validation of stability indicating RP-HPLC method for the determination of ezetimibe in pharmaceutical dosage forms

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