Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis

A simple, efficient and reliable capillary zone electrophoresis method with diode array detection was developed and validated for the simultaneous determination of olmesartan medoxomil and amlodipine besylate in their binary mixtures. The optimum separation for these compounds was achieved with a fu...

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Autores principales: Çelebier, Mustafa, Süslü, Incilay, Altinöz, Sacide
Formato: Articulo
Lenguaje:Español
Publicado: 2011
Materias:
Farmacia
amlodipine besylate; capillary zone electrophoresis; olmesartan medoxomil; pharmaceutical formulations; simultaneous determination
Electroforesis Capilar
Técnicas y Procedimientos de Laboratorio
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8210
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_18.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8210
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Español
topic Farmacia
amlodipine besylate; capillary zone electrophoresis; olmesartan medoxomil; pharmaceutical formulations; simultaneous determination
Electroforesis Capilar
Técnicas y Procedimientos de Laboratorio
spellingShingle Farmacia
amlodipine besylate; capillary zone electrophoresis; olmesartan medoxomil; pharmaceutical formulations; simultaneous determination
Electroforesis Capilar
Técnicas y Procedimientos de Laboratorio
Çelebier, Mustafa
Süslü, Incilay
Altinöz, Sacide
Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
topic_facet Farmacia
amlodipine besylate; capillary zone electrophoresis; olmesartan medoxomil; pharmaceutical formulations; simultaneous determination
Electroforesis Capilar
Técnicas y Procedimientos de Laboratorio
description A simple, efficient and reliable capillary zone electrophoresis method with diode array detection was developed and validated for the simultaneous determination of olmesartan medoxomil and amlodipine besylate in their binary mixtures. The optimum separation for these compounds was achieved with a fused - silica capillary column (i.d. 75.0 µm, total length 48.5 cm and effective length 40.0 cm) and 40.0 mM citrate buffer at pH 6.0 as the running buffer. The samples were injected hydrodynamically for 3 s at 50 mbar and applied voltage was + 15 kV at 30 °C capillary temperature. Detection wavelength was set at 235 nm. Valsartan was used as internal standard. The method was validated with respect to stability, linearity, sensitivity, precision, accuracy, recovery and selectivity. The linear calibration range was found to be 2.00 - 30.00 µg/mL for olmesartan medoxomil and amlodipine besylate. The limits of detection (LOD) were 0.05 µg/mL for both compounds. The relative standard deviations (RSD) of the proposed method ranged between 1.51 and 2.49 % for intra-day precision and 1.51 and 3.73 % for inter-day precision. The developed and validated method successfully applied for the simultaneous determination of olmesartan medoxomil and amlodipine besylate in their pharmaceutical formulations.
format Articulo
Articulo
author Çelebier, Mustafa
Süslü, Incilay
Altinöz, Sacide
author_facet Çelebier, Mustafa
Süslü, Incilay
Altinöz, Sacide
author_sort Çelebier, Mustafa
title Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
title_short Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
title_full Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
title_fullStr Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
title_full_unstemmed Simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
title_sort simultaneous determination of olmesartan medoxomil and amlodipine besylate in pharmaceutical formulations by capillary zone electrophoresis
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8210
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_18.pdf
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