Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography

The aim of this study was to develop and validate selective and sensitive methods for quantitative determination of an antibacterial agent, gemifloxacin, in tablets by high performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE). The HPLC method was carried out on a LiChros...

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Autores principales: Tavares, Vanessa F., Patto, Daniela C.S., Singh, Anil K., Prado, Maria S. A., Kedor-Hackmann, Erika R.M., Santoro, Maria Ines Rocha Miritello
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Agentes Antibacterianos
capillary zone electrophoresis; high performance liquid chromatography; gemifloxacin; tablets; method validation
Técnicas de Química Analítica
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8209
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_17.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8209
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Agentes Antibacterianos
capillary zone electrophoresis; high performance liquid chromatography; gemifloxacin; tablets; method validation
Técnicas de Química Analítica
spellingShingle Farmacia
Agentes Antibacterianos
capillary zone electrophoresis; high performance liquid chromatography; gemifloxacin; tablets; method validation
Técnicas de Química Analítica
Tavares, Vanessa F.
Patto, Daniela C.S.
Singh, Anil K.
Prado, Maria S. A.
Kedor-Hackmann, Erika R.M.
Santoro, Maria Ines Rocha Miritello
Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
topic_facet Farmacia
Agentes Antibacterianos
capillary zone electrophoresis; high performance liquid chromatography; gemifloxacin; tablets; method validation
Técnicas de Química Analítica
description The aim of this study was to develop and validate selective and sensitive methods for quantitative determination of an antibacterial agent, gemifloxacin, in tablets by high performance liquid chromatography (HPLC) and capillary zone electrophoresis (CZE). The HPLC method was carried out on a LiChrospher® 100 RP-8e, 5 μm (125 x 4 mm) column with a mobile phase composed of tetrahydrofuranwater (25:75, v/v) with 0.5 % of triethylamine and pH adjusted to 3.0 with orthophosphoric acid. The CZE method was performed using 50 mM sodium tetraborate buffer (pH 8.6). Samples were injected hydrodynamicaly (0.5 psi, 5 s) and the electrophoretic system was operated under normal polarity, at +20 kV and capillary temperature of 18 ºC. A fused-silica capillary 40.2 cm (30 cm effective length) x 75 μm i.d. was used. Both, HPLC and CZE could be interesting and efficient techniques to be applied for quality control in pharmaceutical industries.
format Articulo
Articulo
author Tavares, Vanessa F.
Patto, Daniela C.S.
Singh, Anil K.
Prado, Maria S. A.
Kedor-Hackmann, Erika R.M.
Santoro, Maria Ines Rocha Miritello
author_facet Tavares, Vanessa F.
Patto, Daniela C.S.
Singh, Anil K.
Prado, Maria S. A.
Kedor-Hackmann, Erika R.M.
Santoro, Maria Ines Rocha Miritello
author_sort Tavares, Vanessa F.
title Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
title_short Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
title_full Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
title_fullStr Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
title_full_unstemmed Quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
title_sort quantitative determination of gemifloxacin mesylate in tablets by capillary zone electrophoresis and high performance liquid chromatography
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8209
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_17.pdf
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