Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD

A simple and rapid SPE-HPLC-DAD method was developed for the determination of 5-fluorouracil (5-FU) in surface samples. A C18 column (250 x 4.6 mm i.d., 5 µm) and a similar pre-column were used for the separation at 25 ºC, using 20 mM ammonium acetate buffer solution pH 4.7: methanol (95:5, v/v) a...

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Detalles Bibliográficos
Autores principales:	Resende, Renata C., Tahan, Gabriella P., Oliveira, Antônio F.F., Maia, Patrícia P., Martins, Isarita
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia Cromatografia líquida de alta eficiencia Pirimidinonas antineoplastic drugs; 5-fluorouracil; occupational exposure; SPE-HPLC-DAD; surface samples
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8208 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_16.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia Cromatografia líquida de alta eficiencia Pirimidinonas antineoplastic drugs; 5-fluorouracil; occupational exposure; SPE-HPLC-DAD; surface samples
spellingShingle	Farmacia Cromatografia líquida de alta eficiencia Pirimidinonas antineoplastic drugs; 5-fluorouracil; occupational exposure; SPE-HPLC-DAD; surface samples Resende, Renata C. Tahan, Gabriella P. Oliveira, Antônio F.F. Maia, Patrícia P. Martins, Isarita Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD
topic_facet	Farmacia Cromatografia líquida de alta eficiencia Pirimidinonas antineoplastic drugs; 5-fluorouracil; occupational exposure; SPE-HPLC-DAD; surface samples
description	A simple and rapid SPE-HPLC-DAD method was developed for the determination of 5-fluorouracil (5-FU) in surface samples. A C18 column (250 x 4.6 mm i.d., 5 µm) and a similar pre-column were used for the separation at 25 ºC, using 20 mM ammonium acetate buffer solution pH 4.7: methanol (95:5, v/v) as mobile phase at a flow rate of 1.2 mL/min. Under optimal conditions, the linearity was 0.9993, in a range of 25-100 µg/mL. The limits of detection and quantification were 5 and 25 µg/mL, respectively. The relative standard deviation (%) was below 15 % for the evaluation of precision and the mean recovery was 77 %. The extracting procedure followed HPLC analysis showed their applicability in order to examine 5- FU in surfaces samples. Moreover, it could be suggested that the developed method is an alternative in the monitoring of the occupational exposure to antineoplastic agents, once the analyte in question is considered an indicator for this purpose.
format	Articulo Articulo
author	Resende, Renata C. Tahan, Gabriella P. Oliveira, Antônio F.F. Maia, Patrícia P. Martins, Isarita
author_facet	Resende, Renata C. Tahan, Gabriella P. Oliveira, Antônio F.F. Maia, Patrícia P. Martins, Isarita
author_sort	Resende, Renata C.
title	Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD
title_short	Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD
title_full	Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD
title_fullStr	Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD
title_full_unstemmed	Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD
title_sort	determination of 5-fluorouracil in surface samples using spe combined with hplc- dad
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8208 http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_16.pdf
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bdutipo_str	Repositorios
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Determination of 5-fluorouracil in surface samples using SPE combined with HPLC- DAD

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