TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations

The poly herbal formulations containing ginger are widely used for different medicinal properties. The present paper deals with the development of densitometric method for fingerprint of ginger mainly using 6-gingerol in the form of densitogram following charring of the chromatographic as internal s...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Shankar, Shukla S., Swarnlata, Saraf, Shailendra, Saraf
Formato: Articulo
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
Técnicas de Química Analítica
ayurvedic formulation; densitometry; fingerprint; 6-gingerol; HPTLC
Preparaciones de Plantas
Preparaciones Farmacéuticas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8196
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_4.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8196
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Técnicas de Química Analítica
ayurvedic formulation; densitometry; fingerprint; 6-gingerol; HPTLC
Preparaciones de Plantas
Preparaciones Farmacéuticas
spellingShingle Farmacia
Técnicas de Química Analítica
ayurvedic formulation; densitometry; fingerprint; 6-gingerol; HPTLC
Preparaciones de Plantas
Preparaciones Farmacéuticas
Shankar, Shukla S.
Swarnlata, Saraf
Shailendra, Saraf
TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
topic_facet Farmacia
Técnicas de Química Analítica
ayurvedic formulation; densitometry; fingerprint; 6-gingerol; HPTLC
Preparaciones de Plantas
Preparaciones Farmacéuticas
description The poly herbal formulations containing ginger are widely used for different medicinal properties. The present paper deals with the development of densitometric method for fingerprint of ginger mainly using 6-gingerol in the form of densitogram following charring of the chromatographic as internal standard that could be useful for marker-based quality assurance of the poly herbal products containing Zingiber officinale. The fast rapid and reproducible method was developed. The accuracy of method was validated by analysis of spiked blank and standard addition samples and precision by performing replicate analyses on a single day and on different days. Recoveries from spiked blank and standard addition sample were 98.03-99.47 %. Repeatability for sample, each of which was analyzed six times on a single plate, was 0.001903 % relative standard deviation. The intra and inter precision was 0.001242 and 0.010676 % relative standard deviation for a sample analyzed in duplicate once per plate on same day and the different days over a seven-day period. The content of 6-Gingerol was found to be 0.698 ± 0.000577, 0.689 ± 0.002, 0.687 ± 0.001528 mg/g in lab formulations. The method was shown to be simple, precise and accurate suitable for routine analysis of 6-Gingerol at different levels from raw material to finished formulations.
format Articulo
Articulo
author Shankar, Shukla S.
Swarnlata, Saraf
Shailendra, Saraf
author_facet Shankar, Shukla S.
Swarnlata, Saraf
Shailendra, Saraf
author_sort Shankar, Shukla S.
title TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
title_short TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
title_full TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
title_fullStr TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
title_full_unstemmed TLC densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
title_sort tlc densitometric fingerprint development and validation of 6-gingerol as marker in poly-herbal ayurvedic formulations
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8196
http://www.latamjpharm.org/resumenes/30/4/LAJOP_30_4_1_4.pdf
work_keys_str_mv AT shankarshuklas tlcdensitometricfingerprintdevelopmentandvalidationof6gingerolasmarkerinpolyherbalayurvedicformulations
AT swarnlatasaraf tlcdensitometricfingerprintdevelopmentandvalidationof6gingerolasmarkerinpolyherbalayurvedicformulations
AT shailendrasaraf tlcdensitometricfingerprintdevelopmentandvalidationof6gingerolasmarkerinpolyherbalayurvedicformulations
bdutipo_str Repositorios
_version_ 1764820488557690881

Ejemplares similares