Microbiological assay for apramycin soluble powder

The aim of this study was to validate an agar diffusion method through the parameters linearity, precision and accuracy, to quantify apramycin in soluble powder. The calibration curve of apramycin was constructed by plotting log of concentrations (µg ml<sup>-1</sup>) versus zone diamete...

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Detalles Bibliográficos
Autores principales: Lourenço, Felipe R., Barbosa, Elisabete A., Pinto, Terezinha J. A.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2011
Materias:
Farmacia
agar diffusion method; apramycin; microbiological assay
Técnicas y Procedimientos de Laboratorio
Pruebas de Sensibilidad Microbiana
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8178
http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_2_3.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8178
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
agar diffusion method; apramycin; microbiological assay
Técnicas y Procedimientos de Laboratorio
Pruebas de Sensibilidad Microbiana
spellingShingle Farmacia
agar diffusion method; apramycin; microbiological assay
Técnicas y Procedimientos de Laboratorio
Pruebas de Sensibilidad Microbiana
Lourenço, Felipe R.
Barbosa, Elisabete A.
Pinto, Terezinha J. A.
Microbiological assay for apramycin soluble powder
topic_facet Farmacia
agar diffusion method; apramycin; microbiological assay
Técnicas y Procedimientos de Laboratorio
Pruebas de Sensibilidad Microbiana
description The aim of this study was to validate an agar diffusion method through the parameters linearity, precision and accuracy, to quantify apramycin in soluble powder. The calibration curve of apramycin was constructed by plotting log of concentrations (µg ml<sup>-1</sup>) versus zone diameter (mm) and shows good linearity in the range of 1.0-4.0 µg ml<sup>-1</sup>. The precision of the assay was determined by assaying samples at the same day (repeatability - R.S.D. = 2.00%) and on different days (intermediate precision - R.S.D. = 5.06%) and indicate good precision. The accuracy expresses the agreement between the accepted value and the value found. The mean recovery was found to be 100.49 % for apramycin soluble powder. The results indicated that the microbiological assay proposed in this work hold linearity, precision and accuracy being an acceptable alternative method for routine quality control of apramycin in the pharmaceutical dosage form studied.
format Articulo
Comunicacion
author Lourenço, Felipe R.
Barbosa, Elisabete A.
Pinto, Terezinha J. A.
author_facet Lourenço, Felipe R.
Barbosa, Elisabete A.
Pinto, Terezinha J. A.
author_sort Lourenço, Felipe R.
title Microbiological assay for apramycin soluble powder
title_short Microbiological assay for apramycin soluble powder
title_full Microbiological assay for apramycin soluble powder
title_fullStr Microbiological assay for apramycin soluble powder
title_full_unstemmed Microbiological assay for apramycin soluble powder
title_sort microbiological assay for apramycin soluble powder
publishDate 2011
url http://sedici.unlp.edu.ar/handle/10915/8178
http://www.latamjpharm.org/resumenes/30/3/LAJOP_30_3_2_3.pdf
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