Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD

A reverse phase high-performance liquid chromatography-photodiode array detector (RP HPLC-DAD) method for simultaneous determination of lamivudine (3TC), zidovudine (AZT), two nucleoside reverse transcriptase inhibitors, and efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor, in table...

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Detalles Bibliográficos
Autores principales:	Soares, Mônica F.L.R., Soares Sobrinho, José L., Silva, Keyla E.R. da, Rolim, Larissa A., Alves, Lariza D.S., Rolim Neto, Pedró José
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia analytical method; efavirenz; lamivudine; RP HPLC-DAD; validation; zidovudine Nucleósidos Técnicas de Investigación
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8134 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_1_9.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

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record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia analytical method; efavirenz; lamivudine; RP HPLC-DAD; validation; zidovudine Nucleósidos Técnicas de Investigación
spellingShingle	Farmacia analytical method; efavirenz; lamivudine; RP HPLC-DAD; validation; zidovudine Nucleósidos Técnicas de Investigación Soares, Mônica F.L.R. Soares Sobrinho, José L. Silva, Keyla E.R. da Rolim, Larissa A. Alves, Lariza D.S. Rolim Neto, Pedró José Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
topic_facet	Farmacia analytical method; efavirenz; lamivudine; RP HPLC-DAD; validation; zidovudine Nucleósidos Técnicas de Investigación
description	A reverse phase high-performance liquid chromatography-photodiode array detector (RP HPLC-DAD) method for simultaneous determination of lamivudine (3TC), zidovudine (AZT), two nucleoside reverse transcriptase inhibitors, and efavirenz (EFV), a non-nucleoside reverse transcriptase inhibitor, in tablets is described. The drugs separation was performed on a C18 column (250 x 4,6 mm, 5 μm - Phenomenex®), through a mobile phase drag composed by a binary gradient of acetonitrile and water HPLC grade. The drugs detection was performed at 248 nm, resulting in a 14 min chromatographic run. The samples were prepared by crushing tablets, weighing powder, diluting it by a 10 min sonication and filtrating solution, resulting in samples with final concentration of 20, 40 e 40 μg.mL–1 of 3TC, AZT and EFV, respectively. The method was adequate for quality control purposes and was validated following the ICH guidelines.
format	Articulo Articulo
author	Soares, Mônica F.L.R. Soares Sobrinho, José L. Silva, Keyla E.R. da Rolim, Larissa A. Alves, Lariza D.S. Rolim Neto, Pedró José
author_facet	Soares, Mônica F.L.R. Soares Sobrinho, José L. Silva, Keyla E.R. da Rolim, Larissa A. Alves, Lariza D.S. Rolim Neto, Pedró José
author_sort	Soares, Mônica F.L.R.
title	Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
title_short	Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
title_full	Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
title_fullStr	Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
title_full_unstemmed	Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD
title_sort	simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by rp hplc-dad
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8134 http://www.latamjpharm.org/resumenes/30/2/LAJOP_30_2_1_9.pdf
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Simultaneous determination of antiretroviral zidovudine, lamivudine and efavirenz by RP HPLC-DAD

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