Biodegradable porous silk microtubes for tissue vascularization
Cardiovascular diseases are the leading cause of mortality around the globe, and microvasculature replacements to help stem these diseases are not available. Additionally, some vascular surgeries needing small-diameter vascular grafts present different performance requirements. In this work, silk fi...
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| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/81258 |
| Aporte de: |
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I19-R120-10915-81258 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Química silk fibroin scaffolds microtubes polyethylene oxide cardiovascular diseases |
| spellingShingle |
Química silk fibroin scaffolds microtubes polyethylene oxide cardiovascular diseases Bosio, Valeria Elizabeth Brown, J. Rodriguez, M. J. Kaplan, D. L. Biodegradable porous silk microtubes for tissue vascularization |
| topic_facet |
Química silk fibroin scaffolds microtubes polyethylene oxide cardiovascular diseases |
| description |
Cardiovascular diseases are the leading cause of mortality around the globe, and microvasculature replacements to help stem these diseases are not available. Additionally, some vascular surgeries needing small-diameter vascular grafts present different performance requirements. In this work, silk fibroin scaffolds based on silk/polyethylene oxide blends were developed as microtubes for vasculature needs and for different tissue regeneration times, mechanical properties and structural designs. Systems with 13, 14 and 15% silk alone or blended with 1 or 2% of polyethylene oxide (PEO) were used to generate porous microtubes by gel spinning. Microtubes with inner diameters (IDs) of 150–300 mm and 100 mm wall thicknesses were fabricated. The systems were assessed for porosity, mechanical properties, enzymatic degradability, and in vitro vascular endothelial cell attachment and metabolic activity. After 14 days, all the tubes supported the proliferation of cells and the cell attachment increased with porosity.
The silk tubes with PEO had similar crystallinity but a higher elastic modulus compared with the systems without PEO. The silk (13%)/PEO (1%) system showed the highest porosity (20 um pore diameter on average), the highest cell attachment and the fastest degradation profile. There was a good correlation between these parameters with silk concentration and the presence of PEO. The results demonstrate the ability to generate versatile and tunable tubular biomaterials based on silk–PEO blends with potential for microvascular grafts. |
| format |
Articulo Articulo |
| author |
Bosio, Valeria Elizabeth Brown, J. Rodriguez, M. J. Kaplan, D. L. |
| author_facet |
Bosio, Valeria Elizabeth Brown, J. Rodriguez, M. J. Kaplan, D. L. |
| author_sort |
Bosio, Valeria Elizabeth |
| title |
Biodegradable porous silk microtubes for tissue vascularization |
| title_short |
Biodegradable porous silk microtubes for tissue vascularization |
| title_full |
Biodegradable porous silk microtubes for tissue vascularization |
| title_fullStr |
Biodegradable porous silk microtubes for tissue vascularization |
| title_full_unstemmed |
Biodegradable porous silk microtubes for tissue vascularization |
| title_sort |
biodegradable porous silk microtubes for tissue vascularization |
| publishDate |
2017 |
| url |
http://sedici.unlp.edu.ar/handle/10915/81258 |
| work_keys_str_mv |
AT bosiovaleriaelizabeth biodegradableporoussilkmicrotubesfortissuevascularization AT brownj biodegradableporoussilkmicrotubesfortissuevascularization AT rodriguezmj biodegradableporoussilkmicrotubesfortissuevascularization AT kaplandl biodegradableporoussilkmicrotubesfortissuevascularization |
| bdutipo_str |
Repositorios |
| _version_ |
1764820487907573760 |