Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma

A micellar liquid chromatographic method was developed for the simultaneous analysis of a binary mixture of norfloxacin and tinidazole (NOR and TIN) in dosage forms and human plasma. The analysis was carried out using a Waters Symmetry® C18 column (250 mm x 4.6 mm i.d., 5 µm particle size). The runn...

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Detalles Bibliográficos
Autores principales:	Walash, M. I., Sharaf El Din, M., El-Enany, N., Eid, M., Shalan, Sh.
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia human plasma; micellar liquid chromatography; norfloxacin; pharmaceutical tablets; tinidazole Cromatografía Liquida Monitoreo de Drogas
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8096 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_4.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8096
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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia human plasma; micellar liquid chromatography; norfloxacin; pharmaceutical tablets; tinidazole Cromatografía Liquida Monitoreo de Drogas
spellingShingle	Farmacia human plasma; micellar liquid chromatography; norfloxacin; pharmaceutical tablets; tinidazole Cromatografía Liquida Monitoreo de Drogas Walash, M. I. Sharaf El Din, M. El-Enany, N. Eid, M. Shalan, Sh. Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
topic_facet	Farmacia human plasma; micellar liquid chromatography; norfloxacin; pharmaceutical tablets; tinidazole Cromatografía Liquida Monitoreo de Drogas
description	A micellar liquid chromatographic method was developed for the simultaneous analysis of a binary mixture of norfloxacin and tinidazole (NOR and TIN) in dosage forms and human plasma. The analysis was carried out using a Waters Symmetry® C18 column (250 mm x 4.6 mm i.d., 5 µm particle size). The running mobile phase consisting of 0.15 M sodium dodecyl sulphate (SDS), 0.3 % triethylamine (TEA), 5 % n-propanol, the pH was adjusted to 4 by addition of 0.02 M orthophosphoric acid pumped at a flow rate 1.0 mL/min with UV at 275 nm. Calibration curves were linear over the range 1-28 and 1.5-42 µg/mL for NOR and TIN, respectively. The quantification limits were 0.7 and 1.0 µg /mL for NOR and TIN respectively. The proposed method was successfully applied for the simultaneous determination of NOR and TIN in human plasma without prior precipitation of protein. The mean percentage recoveries of bioavailability test in human plasma (n = 3) were 90.31 ± 4.22 and 90.05 ± 1.3 for NOR and TIN, respectively.
format	Articulo Articulo
author	Walash, M. I. Sharaf El Din, M. El-Enany, N. Eid, M. Shalan, Sh.
author_facet	Walash, M. I. Sharaf El Din, M. El-Enany, N. Eid, M. Shalan, Sh.
author_sort	Walash, M. I.
title	Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
title_short	Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
title_full	Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
title_fullStr	Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
title_full_unstemmed	Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
title_sort	micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8096 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_4.pdf
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bdutipo_str	Repositorios
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Micellar liquid chromatographic method for the simultaneous determination of norfloxacin and tinidazole in pharmaceutical dosage forms and human plasma

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