Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study

A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion hydrochloride in rat plasma using triazolam as an internal standard. Chromatographic separation was achieved on a SB-C18 column at 30 °C, with 50: 50 (v/v) aceton...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hu, Lufeng, Lin, Guanyang, Wang, Xianqin, Xu, Ren-ai, Zhang, Xiuhua, Zhang, Chunhong
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2011
Materias:	Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/8094 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_2.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-8094
record_format	dspace
institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas
spellingShingle	Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas Hu, Lufeng Lin, Guanyang Wang, Xianqin Xu, Ren-ai Zhang, Xiuhua Zhang, Chunhong Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
topic_facet	Farmacia bupropion hydrochloride; LC-MS/MS; rat plasma Cromatografía líquida Fenetilaminas Farmacocinética Ratas
description	A selective and sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for quantitation of bupropion hydrochloride in rat plasma using triazolam as an internal standard. Chromatographic separation was achieved on a SB-C18 column at 30 °C, with 50: 50 (v/v) acetonitrile-0.1 % formic acid in water as mobile phase. The flow rate was 0.3 mL/min. The determination of bupropion was performed in MRM mode, m/z 239.9 → 183.7 for bupropion and m/z 343.0 → 308.0 for triazolam (IS) and positive ion electrospray ionization interface. Calibration curve was linear over range of 1.2 to 480 ng/mL. The intra- and inter-run relative standard deviations of the assay were less than 10 %. The mean absolute recoveries determined at the concentrations of 2.4, 48 and 360 ng/mLwere 91.00%, 92.06%, 91.71%, respectively. The validated method is successfully used to analyze the drug in samples of rat plasma for pharmacokinetic study.
format	Articulo Articulo
author	Hu, Lufeng Lin, Guanyang Wang, Xianqin Xu, Ren-ai Zhang, Xiuhua Zhang, Chunhong
author_facet	Hu, Lufeng Lin, Guanyang Wang, Xianqin Xu, Ren-ai Zhang, Xiuhua Zhang, Chunhong
author_sort	Hu, Lufeng
title	Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
title_short	Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
title_full	Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
title_fullStr	Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
title_full_unstemmed	Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study
title_sort	determination of bupropion hydrochloride in rat plasma by lc-ms/ms and its application to pharmacokinetic study
publishDate	2011
url	http://sedici.unlp.edu.ar/handle/10915/8094 http://www.latamjpharm.org/resumenes/30/1/LAJOP_30_1_1_2.pdf
work_keys_str_mv	AT hulufeng determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT linguanyang determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT wangxianqin determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT xurenai determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT zhangxiuhua determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy AT zhangchunhong determinationofbupropionhydrochlorideinratplasmabylcmsmsanditsapplicationtopharmacokineticstudy
bdutipo_str	Repositorios
_version_	1764820487899185152

Determination of bupropion hydrochloride in rat plasma by LC-MS/MS and Its application to pharmacokinetic study

Ejemplares similares