Antioxidant properties of polyphenol-rich cocoa products industrially processed

Fermentation and roasting are the main causes of polyphenol degradation during the process for obtaining cocoa products. In the present study, a process for obtaining polyphenol-rich cocoa products on an industrial scale is described. The process avoids the fermentation and roasting steps and includ...

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Autores principales: Schinella, Guillermo Raúl, Mosca, Susana María, Cienfuegos Jovellanos, Elena, Pasamar, María Ángeles, Muguerza, Begoña, Ramón, Daniel, Ríos, José Luis
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Ciencias Exactas
Theobroma cacao
Cocoa
Antioxidant
Polyphenols
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/80926
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-80926
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Ciencias Exactas
Theobroma cacao
Cocoa
Antioxidant
Polyphenols
spellingShingle Ciencias Exactas
Theobroma cacao
Cocoa
Antioxidant
Polyphenols
Schinella, Guillermo Raúl
Mosca, Susana María
Cienfuegos Jovellanos, Elena
Pasamar, María Ángeles
Muguerza, Begoña
Ramón, Daniel
Ríos, José Luis
Antioxidant properties of polyphenol-rich cocoa products industrially processed
topic_facet Ciencias Exactas
Theobroma cacao
Cocoa
Antioxidant
Polyphenols
description Fermentation and roasting are the main causes of polyphenol degradation during the process for obtaining cocoa products. In the present study, a process for obtaining polyphenol-rich cocoa products on an industrial scale is described. The process avoids the fermentation and roasting steps and includes a step for the inactivation of the enzyme Polyphenol Oxidase (PPO), which helps preserve the polyphenol content present in the raw cocoa bean. In addition, our study evaluates the antioxidant capacity and characterizes the flavonoid profile of the polyphenol-rich cocoa products obtained from the natural polyphenolrich cocoa cake. Using different protocols, we have obtained three cocoa extracts with high polyphenol content, namely extracts A (167 mg/g), B (374 mg/g) and C (787 mg/g). The scavenging capacity of the extracts was measured as their ability to bleach the stable radicals DPPH and ABTS + while their antioxidant effect was evaluated with the FRAP assay. The results for A, B and C in the DPPH test expressed as Trolox equivalent (lmol)/mg dry weight of extract were 0.2, 1.4 and 3.0, respectively; in the ABTS test the results were 1.0, 4.7 and 9.8. The antioxidant capacity expressed as ascorbic acid equivalent (lmol)/mg dry weight of each product were 17.2, 76.1 and 207.7, respectively. The scavenging properties of cocoa powder against the superoxide anion, H2O2, HClO, and peroxynitrite were also determined. The IC50 (lg/mL) values in the hypoxanthine/xanthine oxidase test were 77.5, 12.3 and 10.3, for A, B and C, respectively, while as an HOCl scavenger the IC50 (lg/mL) values were 225.4, 73.2 and 21.5. As a peroxynitrite anion scavenger, only extract C had a relevant effect, with IC50 (lg/mL) values of 76.1 or 110.0 in the absence or presence of bicarbonate. None of the extracts tested showed activity in the hydrogen peroxide test, but B and C significantly increased the deoxyribose degradation in the absence of ascorbate. Likewise, none of the extracts inhibited the ferrous or copper chelating activity at 100 lg/mL, but they inhibited the lipid peroxidation in brain homogenates and human plasma through non-enzymatic generation systems, with extract C giving the best IC50 (lg/mL) values: 17.4 and 8.1 against lipid peroxidation in brain homogenates and human plasma, respectively. In conclusion, if the extractive protocol is well characterized, defined and optimized, cocoa could constitute a source of polyphenols for enriching foods, nutraceuticals and alimentary supplements.
format Articulo
Articulo
author Schinella, Guillermo Raúl
Mosca, Susana María
Cienfuegos Jovellanos, Elena
Pasamar, María Ángeles
Muguerza, Begoña
Ramón, Daniel
Ríos, José Luis
author_facet Schinella, Guillermo Raúl
Mosca, Susana María
Cienfuegos Jovellanos, Elena
Pasamar, María Ángeles
Muguerza, Begoña
Ramón, Daniel
Ríos, José Luis
author_sort Schinella, Guillermo Raúl
title Antioxidant properties of polyphenol-rich cocoa products industrially processed
title_short Antioxidant properties of polyphenol-rich cocoa products industrially processed
title_full Antioxidant properties of polyphenol-rich cocoa products industrially processed
title_fullStr Antioxidant properties of polyphenol-rich cocoa products industrially processed
title_full_unstemmed Antioxidant properties of polyphenol-rich cocoa products industrially processed
title_sort antioxidant properties of polyphenol-rich cocoa products industrially processed
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/80926
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bdutipo_str Repositorios
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