HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets

A new simple, rapid and reproducible high performance thin layer chromatographic method has been developed and validated for the analysis of granisetron hydrochloride (GSH) in bulk drug and from pharmaceutical formulation. The chromatographic separation was achieved on HPTLC aluminium plates precoat...

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Autores principales: Lakshmana Prabu, S., Selvamani, P., Latha, S.
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
bulk drug; granisetron hydrochloride; HPTLC; tablets
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8089
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_2_6.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8089
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
bulk drug; granisetron hydrochloride; HPTLC; tablets
spellingShingle Farmacia
bulk drug; granisetron hydrochloride; HPTLC; tablets
Lakshmana Prabu, S.
Selvamani, P.
Latha, S.
HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
topic_facet Farmacia
bulk drug; granisetron hydrochloride; HPTLC; tablets
description A new simple, rapid and reproducible high performance thin layer chromatographic method has been developed and validated for the analysis of granisetron hydrochloride (GSH) in bulk drug and from pharmaceutical formulation. The chromatographic separation was achieved on HPTLC aluminium plates precoated with silica gel 60F<sub>254</sub> as the stationary phase with chloroform: methanol (80:20 %v/v) as mobile phase. The method gives a compact band for GSH (Rf value of 0.45 ± 0.02). Densitometric analysis of GSH was carried out in the absorbance mode at 301 nm. The linear regression analysis data for the calibration plots showed good linear relationship with the correlation coefficient of 0.9980 with respect to peak area in the concentration range of 400-1600 ngband<sup>-1</sup>. The method was validated for accuracy, precision, recovery studies, specificity, sensitivity, limit of detection and limit of quantification. Statistical analysis proved the method was precise, reproducible, selective, specific, and accurate for analysis of GSH. The wide linearity range, sensitivity, accuracy, and simple mobile phase composition imply the method is suitable for routine quantification of GSH with high precision and accuracy in bulk drug and marketed oral solid dosage form.
format Articulo
Comunicacion
author Lakshmana Prabu, S.
Selvamani, P.
Latha, S.
author_facet Lakshmana Prabu, S.
Selvamani, P.
Latha, S.
author_sort Lakshmana Prabu, S.
title HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
title_short HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
title_full HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
title_fullStr HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
title_full_unstemmed HPTLC method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
title_sort hptlc method for quantitative determination of granisetron hydrochloride in bulk drug and in tablets
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8089
http://www.latamjpharm.org/resumenes/29/8/LAJOP_29_8_2_6.pdf
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AT lathas hptlcmethodforquantitativedeterminationofgranisetronhydrochlorideinbulkdrugandintablets
bdutipo_str Repositorios
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