Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine

In the present study, a high performance liquid chromatographic method was developed and validated for determination of chromatographic purity and stress stability of the anti-retroviral drug lamivudine. The different analytical performance parameters such as linearity, precision, specificity, limit...

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Autores principales: Kakkar, Saloni, Mohanraj, Krishnapriya, Deep, Aakash, Sharma, Prabodh C.
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
chromatographic purity; lamivudine; RP-HPLC; stress stability; validation
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/8030
http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_3.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-8030
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
chromatographic purity; lamivudine; RP-HPLC; stress stability; validation
spellingShingle Farmacia
chromatographic purity; lamivudine; RP-HPLC; stress stability; validation
Kakkar, Saloni
Mohanraj, Krishnapriya
Deep, Aakash
Sharma, Prabodh C.
Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine
topic_facet Farmacia
chromatographic purity; lamivudine; RP-HPLC; stress stability; validation
description In the present study, a high performance liquid chromatographic method was developed and validated for determination of chromatographic purity and stress stability of the anti-retroviral drug lamivudine. The different analytical performance parameters such as linearity, precision, specificity, limit of detection, limit of quantification, robustness and ruggedness were determined according to International Conference on harmonization ICH Q2B guidelines. Chromatography was carried out by gradient technique on a reverse phase C-18 Inertsil ODS 3V using Agilent Chemstation 1200 series equipped with photo diode array detector (λ = 273nm) with mobile phase based and optimized depending on the polarity of the molecule. All the system suitability parameters were found within the range. The proposed method is highly sensitive, precise and hence successfully applied to the chromatographic purity of lamivudine active pharmaceutical ingredient (API).
format Articulo
Articulo
author Kakkar, Saloni
Mohanraj, Krishnapriya
Deep, Aakash
Sharma, Prabodh C.
author_facet Kakkar, Saloni
Mohanraj, Krishnapriya
Deep, Aakash
Sharma, Prabodh C.
author_sort Kakkar, Saloni
title Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine
title_short Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine
title_full Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine
title_fullStr Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine
title_full_unstemmed Simple and rapid RP-HPLC method to determine the purity of the anti-retroviral drug lamivudine
title_sort simple and rapid rp-hplc method to determine the purity of the anti-retroviral drug lamivudine
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/8030
http://www.latamjpharm.org/resumenes/29/7/LAJOP_29_7_1_3.pdf
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