In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test

D-002 is a mixture of 6 high molecular weight primary alcohols purified from beeswax with anti-inflammatory and antioxidant effects. This study investigated the in vitro genotoxic potential of D-002 to produce chromosomal aberrations (CA) in peripheral blood lymphocytes. Fresh suspensions of BWA wer...

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Autores principales: Gámez, Rafael, Gutiérrez, Ariadne, Más, Rosa, Arencibia, Daniel, Curveco, Dayisell, García, Haydée
Formato: Articulo Comunicacion
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
Farmacología
anti-inflammatory; antioxidant; chromosomal aberrations test; d-002; higher aliphatic primary alcohols; Lymphocyte cultures
Antioxidantes
Cromosomas
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7933
http://www.latamjpharm.org/resumenes/29/3/LAJOP_29_3_2_3.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7933
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Farmacología
anti-inflammatory; antioxidant; chromosomal aberrations test; d-002; higher aliphatic primary alcohols; Lymphocyte cultures
Antioxidantes
Cromosomas
spellingShingle Farmacia
Farmacología
anti-inflammatory; antioxidant; chromosomal aberrations test; d-002; higher aliphatic primary alcohols; Lymphocyte cultures
Antioxidantes
Cromosomas
Gámez, Rafael
Gutiérrez, Ariadne
Más, Rosa
Arencibia, Daniel
Curveco, Dayisell
García, Haydée
In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test
topic_facet Farmacia
Farmacología
anti-inflammatory; antioxidant; chromosomal aberrations test; d-002; higher aliphatic primary alcohols; Lymphocyte cultures
Antioxidantes
Cromosomas
description D-002 is a mixture of 6 high molecular weight primary alcohols purified from beeswax with anti-inflammatory and antioxidant effects. This study investigated the in vitro genotoxic potential of D-002 to produce chromosomal aberrations (CA) in peripheral blood lymphocytes. Fresh suspensions of BWA were added (500; 750, 1000, 2500 and 5000 μg/mL) to cultures with (microsomal liver fraction S9 mix) or without metabolic activation. Concurrent negative (Tween/water vehicle) and positive controls (cyclophosphamide 15 μg/mL and mitomicyn C (0.3 μg/mL in the tests with and without metabolic activation, respectively) were included. Two hundred (200) metaphases by group were examined and the numbers and frequencies of cells with aberrations and the mitotic index were quantified. D-002 added up to 5 mg/mL of culture did not induce chromosomal aberrations in presence or not of S9 mix compared with negative controls and no trends with the doses were observed. Positive controls evidenced increases on the numbers and frequencies of CA. Mitotic indexes were unchanged with the treatment, which indicates that D-002 does not affect cell proliferation. In conclusion, BWA added in vitro (0.5-5 mg/mL) to peripheral blood lymphocyte cultures did not show evidences of cytotoxic or genotoxic potential in the CA test.
format Articulo
Comunicacion
author Gámez, Rafael
Gutiérrez, Ariadne
Más, Rosa
Arencibia, Daniel
Curveco, Dayisell
García, Haydée
author_facet Gámez, Rafael
Gutiérrez, Ariadne
Más, Rosa
Arencibia, Daniel
Curveco, Dayisell
García, Haydée
author_sort Gámez, Rafael
title In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test
title_short In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test
title_full In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test
title_fullStr In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test
title_full_unstemmed In vitro genotoxic potential of D-002 on the blood lymphocytes chromosomal aberration test
title_sort in vitro genotoxic potential of d-002 on the blood lymphocytes chromosomal aberration test
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7933
http://www.latamjpharm.org/resumenes/29/3/LAJOP_29_3_2_3.pdf
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