Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells

Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 have been used as tools for the screening of anti-inflammatory agents. In a search for inhibitors of COX-2 and iNOS, we found that extracts of Enteromorpha prolifera inhibit the production of nitric oxide (NO) and prostaglandin (PG)E&...

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Detalles Bibliográficos
Autores principales: Yoon, Weon-Jong, Kim, Dong Sam, Yang, Eun-Jin, Moon, Ji-Young, Kim, Min-Jin, Lee, Wook Jae, Lee, Nam Ho, Hyun, Chang-Gu
Formato: Articulo
Lenguaje:Inglés
Publicado: 2010
Materias:
Farmacia
cytokine; enteromorpha prolifera; inflammation; iNOS; PGE2
Agentes antiinflamatorios
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7890
http://www.latamjpharm.org/resumenes/29/2/LAJOP_29_2_1_1.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7890
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
cytokine; enteromorpha prolifera; inflammation; iNOS; PGE2
Agentes antiinflamatorios
spellingShingle Farmacia
cytokine; enteromorpha prolifera; inflammation; iNOS; PGE2
Agentes antiinflamatorios
Yoon, Weon-Jong
Kim, Dong Sam
Yang, Eun-Jin
Moon, Ji-Young
Kim, Min-Jin
Lee, Wook Jae
Lee, Nam Ho
Hyun, Chang-Gu
Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells
topic_facet Farmacia
cytokine; enteromorpha prolifera; inflammation; iNOS; PGE2
Agentes antiinflamatorios
description Inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 have been used as tools for the screening of anti-inflammatory agents. In a search for inhibitors of COX-2 and iNOS, we found that extracts of Enteromorpha prolifera inhibit the production of nitric oxide (NO) and prostaglandin (PG)E<sub>2</sub> in LPS-stimulated RAW 264.7 macrophage cells. We first extracted E. prolifera with 80% ethanol and the extract was partitioned with hexane, dichloromethane, ethyl acetate, butanol, and water, successively. The results indicate that the hexane and ethyl acetate fractions effective inhibited LPS-induced NO and PGE<sub>2</sub> production in RAW 264.7 cells. To test the inhibition effects of the E. prolifera fractions on other cytokines, we also performed an ELISA assay on tumor necrosis factor (TNF)-α, IL-1β, and IL-6 in LPS-stimulated RAW 264.7 macrophage cells. The expression of TNF-α, IL-1β, and IL-6 was also decreased following treatment with the hexane and ethyl acetate fractions. To test the potential application of the E. prolifera extract as a cosmetic material, we also performed MTT assays on keratinocyte HaCaT cells as well as primary skin irritation tests. In these assays, the E. prolifera extracts did not induce any adverse reactions. Based on these results, we suggest that E. prolifera extracts may be considered potential anti-inflammatory candidates for skin health.
format Articulo
Articulo
author Yoon, Weon-Jong
Kim, Dong Sam
Yang, Eun-Jin
Moon, Ji-Young
Kim, Min-Jin
Lee, Wook Jae
Lee, Nam Ho
Hyun, Chang-Gu
author_facet Yoon, Weon-Jong
Kim, Dong Sam
Yang, Eun-Jin
Moon, Ji-Young
Kim, Min-Jin
Lee, Wook Jae
Lee, Nam Ho
Hyun, Chang-Gu
author_sort Yoon, Weon-Jong
title Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells
title_short Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells
title_full Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells
title_fullStr Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells
title_full_unstemmed Inhibitory effects of Enteromorpha prolifera on the production of nitric oxide, prostaglandin E2, and pro-inflammatory cytokines in RAW 264.7 cells
title_sort inhibitory effects of enteromorpha prolifera on the production of nitric oxide, prostaglandin e2, and pro-inflammatory cytokines in raw 264.7 cells
publishDate 2010
url http://sedici.unlp.edu.ar/handle/10915/7890
http://www.latamjpharm.org/resumenes/29/2/LAJOP_29_2_1_1.pdf
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