Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling
The cell viability of Lepechinia speciosa (St. Hill) Epling fractions was measured by cell membrane integrity (lactate dehydrogenase assay) on rat basophilic leukemia cells (RBL-2H3). All fractions and extract tested (100 μg/ml) increased the release of lactate dehydrogenase (LDH), being the ethyl a...
Guardado en:
| Autores principales: | , , , |
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| Formato: | Articulo |
| Lenguaje: | Inglés |
| Publicado: |
2010
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| Materias: | |
| Acceso en línea: | http://sedici.unlp.edu.ar/handle/10915/7868 http://www.latamjpharm.org/resumenes/29/1/LAJOP_29_1_1_5.pdf |
| Aporte de: |
| id |
I19-R120-10915-7868 |
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| record_format |
dspace |
| institution |
Universidad Nacional de La Plata |
| institution_str |
I-19 |
| repository_str |
R-120 |
| collection |
SEDICI (UNLP) |
| language |
Inglés |
| topic |
Farmacia Farmacología cell viability; lamiaceae; lepechinia speciosa; oleanolic acid; ursolic acid Cromatografía |
| spellingShingle |
Farmacia Farmacología cell viability; lamiaceae; lepechinia speciosa; oleanolic acid; ursolic acid Cromatografía Esteves, Patricia Fontes Kuster, Ricardo M. Barbi, Nancy dos S. Menezes, Fabio de S. Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling |
| topic_facet |
Farmacia Farmacología cell viability; lamiaceae; lepechinia speciosa; oleanolic acid; ursolic acid Cromatografía |
| description |
The cell viability of Lepechinia speciosa (St. Hill) Epling fractions was measured by cell membrane integrity (lactate dehydrogenase assay) on rat basophilic leukemia cells (RBL-2H3). All fractions and extract tested (100 μg/ml) increased the release of lactate dehydrogenase (LDH), being the ethyl acetate and dichloromethane fractions with LDH release of 94.5% and 91.2%, respectively. As these fractions showed decrease of cell viability, the antiproliferative activity on human breast adenocarcinoma cells (MCF-7) through sulphorhodamine B (SRB) assay was performed with them. The dichloromethane fraction (50 μg/ml) displayed the maximum activity (95% of inhibition) (IC50 = 1.99 ± 0.06 μg/ml). From this fraction was obtained a mixture containing two triterpenes (ursolic and oleanolic acids) and one fatty acid (palmitic acid), which were identified by gas chromatography coupled to mass spectrometry (GC-MS) and had their structures confirmed by <sub>13</sub>C NMR. Rosmarinic acid and verbascoside were isolated from the ethyl acetate fraction and had their structures confirmed by <sub>1</sub>H NMR. |
| format |
Articulo Articulo |
| author |
Esteves, Patricia Fontes Kuster, Ricardo M. Barbi, Nancy dos S. Menezes, Fabio de S. |
| author_facet |
Esteves, Patricia Fontes Kuster, Ricardo M. Barbi, Nancy dos S. Menezes, Fabio de S. |
| author_sort |
Esteves, Patricia Fontes |
| title |
Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling |
| title_short |
Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling |
| title_full |
Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling |
| title_fullStr |
Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling |
| title_full_unstemmed |
Chemical composition and cytotoxic activity of Lepechinia speciosa (St. Hill) Epling |
| title_sort |
chemical composition and cytotoxic activity of lepechinia speciosa (st. hill) epling |
| publishDate |
2010 |
| url |
http://sedici.unlp.edu.ar/handle/10915/7868 http://www.latamjpharm.org/resumenes/29/1/LAJOP_29_1_1_5.pdf |
| work_keys_str_mv |
AT estevespatriciafontes chemicalcompositionandcytotoxicactivityoflepechiniaspeciosasthillepling AT kusterricardom chemicalcompositionandcytotoxicactivityoflepechiniaspeciosasthillepling AT barbinancydoss chemicalcompositionandcytotoxicactivityoflepechiniaspeciosasthillepling AT menezesfabiodes chemicalcompositionandcytotoxicactivityoflepechiniaspeciosasthillepling |
| bdutipo_str |
Repositorios |
| _version_ |
1764820486460538881 |