Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay

The present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydrop...

Descripción completa

Detalles Bibliográficos
Autores principales: Savignone, César A., Palacios, Alejandro
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/78624
https://medicalresearchjournal.org/index.php/GJMR/article/view/1272
Aporte de:
id I19-R120-10915-78624
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
spellingShingle Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
Savignone, César A.
Palacios, Alejandro
Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
topic_facet Veterinaria
oxidative stress
exercise
chemiluminescence
erythrocyte
tert-butyl hydroperoxide
description The present investigation was carried out to determine the presence of oxidative alterations in the horses erythrocyte membrane during a high intensity exercise test. The degree of peroxidation was estimated by chemiluminescence using a suspension of lysed erythrocytes incubated with t-butyl hydroperoxide (t-BHP). Differences were observed in the total values of chemiluminescence throughout the exercise routine, with higher values of light emission obtained with the animal at rest in relation to those observed during and after exercise.The conclusions of this study are the existence of changes in the erythrocyte membranes of the horses exposed to physical exertion, probably associated with the release of ROS caused by the exercise and that the determination of chemiluminescence in suspension of lysates erythrocyte is a sensitive assay applied to detect the existence of oxidative stress associated to physical exercise.
format Articulo
Articulo
author Savignone, César A.
Palacios, Alejandro
author_facet Savignone, César A.
Palacios, Alejandro
author_sort Savignone, César A.
title Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_short Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_full Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_fullStr Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_full_unstemmed Equine Erythrocyte Lysed Exposed to T-Butyl Hydroperoxide as a Model to Study the Oxidative Stress Caused by Exercise Using a Chemiluminescence Assay
title_sort equine erythrocyte lysed exposed to t-butyl hydroperoxide as a model to study the oxidative stress caused by exercise using a chemiluminescence assay
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/78624
https://medicalresearchjournal.org/index.php/GJMR/article/view/1272
work_keys_str_mv AT savignonecesara equineerythrocytelysedexposedtotbutylhydroperoxideasamodeltostudytheoxidativestresscausedbyexerciseusingachemiluminescenceassay
AT palaciosalejandro equineerythrocytelysedexposedtotbutylhydroperoxideasamodeltostudytheoxidativestresscausedbyexerciseusingachemiluminescenceassay
bdutipo_str Repositorios
_version_ 1764820486343098368