UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets

A stability-indicating high-performance liquid chromatographic (HPLC) and a second-order derivative spectrophotometric (UVDS) analytical methods were validated and compared for determination of simvastatin in tablets. The HPLC method was performed with isocratic elution using a C18 column and a mobi...

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Autores principales: Gomes, Fabio P., García, Pedro L., Alves, João M. P., Singh, Anil K., Kedor-Hackmann, Erika R.M., Santoro, Maria Ines Rocha Miritello
Formato: Articulo
Lenguaje:Inglés
Publicado: 2009
Materias:
Farmacia
Medicamento
simvastatin; stability-indicating HPLC method; UVDS method
Cromatografía líquida
Ciencia. Investigación. Metodología
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/7755
http://www.latamjpharm.org/trabajos/28/2/LAJOP_28_2_1_14_X2H6Z75749.pdf
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-7755
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Farmacia
Medicamento
simvastatin; stability-indicating HPLC method; UVDS method
Cromatografía líquida
Ciencia. Investigación. Metodología
spellingShingle Farmacia
Medicamento
simvastatin; stability-indicating HPLC method; UVDS method
Cromatografía líquida
Ciencia. Investigación. Metodología
Gomes, Fabio P.
García, Pedro L.
Alves, João M. P.
Singh, Anil K.
Kedor-Hackmann, Erika R.M.
Santoro, Maria Ines Rocha Miritello
UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets
topic_facet Farmacia
Medicamento
simvastatin; stability-indicating HPLC method; UVDS method
Cromatografía líquida
Ciencia. Investigación. Metodología
description A stability-indicating high-performance liquid chromatographic (HPLC) and a second-order derivative spectrophotometric (UVDS) analytical methods were validated and compared for determination of simvastatin in tablets. The HPLC method was performed with isocratic elution using a C18 column and a mobile phase composed of methanol:acetonitrile:water (60:20:20, v/v/v) at a flow rate of 1.0 ml/min. The detection was made at 239 nm. In UVDS method, methanol and water were used in first dilution and distilled water was used in consecutive dilutions and as background. The second-order derivative signal measurement was taken at 255 nm. Analytical curves showed correlation coefficients > 0.999 for both methods. The quantitation limits (QL) were 2.41 μg/ml for HPLC and 0.45 μg/ml for UVDS, respectively. Intra and inter-day relative standard deviations were < 2.0 %. Statistical analysis with t- and F-tests are not exceeding their critical values demonstrating that there is no significant difference between the two methods at 95 % confidence level.
format Articulo
Articulo
author Gomes, Fabio P.
García, Pedro L.
Alves, João M. P.
Singh, Anil K.
Kedor-Hackmann, Erika R.M.
Santoro, Maria Ines Rocha Miritello
author_facet Gomes, Fabio P.
García, Pedro L.
Alves, João M. P.
Singh, Anil K.
Kedor-Hackmann, Erika R.M.
Santoro, Maria Ines Rocha Miritello
author_sort Gomes, Fabio P.
title UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets
title_short UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets
title_full UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets
title_fullStr UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets
title_full_unstemmed UV-Derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of Simvastatin in tablets
title_sort uv-derivative spectrophotometric and stability-indicating high-performance liquid chromatographic methods for determination of simvastatin in tablets
publishDate 2009
url http://sedici.unlp.edu.ar/handle/10915/7755
http://www.latamjpharm.org/trabajos/28/2/LAJOP_28_2_1_14_X2H6Z75749.pdf
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