Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method

A reproducible and selective method for the simultaneous determination of cyclophosphamide (CP) and ifosfamide (IF) in plasma has been developed and validated using isocratic elution. The assay is performed by HPLC-UV, with a C18 column (5μm, 150 x 4 mm) and detection in 195 nm. The mobile phase was...

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Detalles Bibliográficos
Autores principales:	Martins, Isarita, Souza, Jeferson O., Sanson, Ananda L., Vieira, Elisabeth, Guisti-Paiva, Alexandre
Formato:	Articulo
Lenguaje:	Inglés
Publicado:	2009
Materias:	Farmacia antineoplastic drugs; HPLC; cyclophosphamide; Ifosfamide; simultaneous determination Productos químicos y drogas Evaluación de drogas Toxicología Preparaciones farmacéuticas
Acceso en línea:	http://sedici.unlp.edu.ar/handle/10915/7723 http://www.latamjpharm.org/trabajos/28/1/LAJOP_28_1_1_6_O9LZ159788.pdf
Aporte de:	SEDICI (UNLP) de Universidad Nacional de La Plata

id	I19-R120-10915-7723
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institution	Universidad Nacional de La Plata
institution_str	I-19
repository_str	R-120
collection	SEDICI (UNLP)
language	Inglés
topic	Farmacia antineoplastic drugs; HPLC; cyclophosphamide; Ifosfamide; simultaneous determination Productos químicos y drogas Evaluación de drogas Toxicología Preparaciones farmacéuticas
spellingShingle	Farmacia antineoplastic drugs; HPLC; cyclophosphamide; Ifosfamide; simultaneous determination Productos químicos y drogas Evaluación de drogas Toxicología Preparaciones farmacéuticas Martins, Isarita Souza, Jeferson O. Sanson, Ananda L. Vieira, Elisabeth Guisti-Paiva, Alexandre Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
topic_facet	Farmacia antineoplastic drugs; HPLC; cyclophosphamide; Ifosfamide; simultaneous determination Productos químicos y drogas Evaluación de drogas Toxicología Preparaciones farmacéuticas
description	A reproducible and selective method for the simultaneous determination of cyclophosphamide (CP) and ifosfamide (IF) in plasma has been developed and validated using isocratic elution. The assay is performed by HPLC-UV, with a C18 column (5μm, 150 x 4 mm) and detection in 195 nm. The mobile phase was constituted by phosphate buffer 10 mM pH 6.0: acetonitrile (77.25:22.75), with a flow of 1 mL/min. SPE was used for sample clean-up in a range from 3 (LOQ) to 540 μM. The repeatability coefficients of variation (CV) ranged from 0.5 to 7.8% and the intermediate precision CVs varied from 0.9 to 5.7%. Extraction efficacy and accuracy varied from 94 to 115%. The simple method may permit the determination of cyclophosphamide and ifosfamide in plasma, simultaneously, to pharmacokinetics and bioequivalence studies.
format	Articulo Articulo
author	Martins, Isarita Souza, Jeferson O. Sanson, Ananda L. Vieira, Elisabeth Guisti-Paiva, Alexandre
author_facet	Martins, Isarita Souza, Jeferson O. Sanson, Ananda L. Vieira, Elisabeth Guisti-Paiva, Alexandre
author_sort	Martins, Isarita
title	Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
title_short	Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
title_full	Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
title_fullStr	Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
title_full_unstemmed	Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method
title_sort	simultaneous determination of cyclophosphamide and ifosfamide in plasma using spe-hplc-uv method
publishDate	2009
url	http://sedici.unlp.edu.ar/handle/10915/7723 http://www.latamjpharm.org/trabajos/28/1/LAJOP_28_1_1_6_O9LZ159788.pdf
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bdutipo_str	Repositorios
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Simultaneous determination of cyclophosphamide and Ifosfamide in plasma using SPE-HPLC-UV method

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