Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins

Plants have evolved sophisticated embryo defences by kinetically-stable non-digestible storage proteins that lower the nutritional value of seeds, a strategy that have not been reported in animals. To further understand antinutritive defences in animals, we analysed PmPV1, massively accumulated in...

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Detalles Bibliográficos
Autores principales: Pasquevich, María Yanina, Dreon, Marcos Sebastián, Qiu, Jian-Wen, Mu, Huawei, Heras, Horacio
Formato: Articulo
Lenguaje:Inglés
Publicado: 2017
Materias:
Bioquímica
Plantas
PmPV1
embryo defences
Acceso en línea:http://sedici.unlp.edu.ar/handle/10915/77105
Aporte de:
SEDICI (UNLP) de Universidad Nacional de La Plata
id I19-R120-10915-77105
record_format dspace
institution Universidad Nacional de La Plata
institution_str I-19
repository_str R-120
collection SEDICI (UNLP)
language Inglés
topic Bioquímica
Plantas
PmPV1
embryo defences
spellingShingle Bioquímica
Plantas
PmPV1
embryo defences
Pasquevich, María Yanina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Mu, Huawei
Heras, Horacio
Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
topic_facet Bioquímica
Plantas
PmPV1
embryo defences
description Plants have evolved sophisticated embryo defences by kinetically-stable non-digestible storage proteins that lower the nutritional value of seeds, a strategy that have not been reported in animals. To further understand antinutritive defences in animals, we analysed PmPV1, massively accumulated in the eggs of the gastropod Pomacea maculata, focusing on how its structure and structural stability features affected its capacity to withstand passage through predator guts. The native protein withstands >50 min boiling and resists the denaturing detergent sodium dodecyl sulphate (SDS), indicating an unusually high structural stability (i.e., kinetic stability). PmPV1 is highly resistant to in vitro proteinase digestion and displays structural stability between pH 2.0–12.0 and 25–85 °C. Furthermore, PmPV1 withstands in vitro and mice digestion and is recovered unchanged in faeces, supporting an antinutritive defensive function. Subunit sequence similarities suggest a common origin and tolerance to mutations. This is the first known animal genus that, like plant seeds, lowers the nutritional value of eggs by kinetically-stable non-digestible storage proteins that survive the gut of predators unaffected. The selective pressure of the harsh gastrointestinal environment would have favoured their appearance, extending by convergent evolution the presence of plant-like hyperstable antinutritive proteins to unattended reproductive stages in animals.
format Articulo
Articulo
author Pasquevich, María Yanina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Mu, Huawei
Heras, Horacio
author_facet Pasquevich, María Yanina
Dreon, Marcos Sebastián
Qiu, Jian-Wen
Mu, Huawei
Heras, Horacio
author_sort Pasquevich, María Yanina
title Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
title_short Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
title_full Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
title_fullStr Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
title_full_unstemmed Convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
title_sort convergent evolution of plant and animal embryo defences by hyperstable non-digestible storage proteins
publishDate 2017
url http://sedici.unlp.edu.ar/handle/10915/77105
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